Primary Cells
Comprehensive Collection of Ethically Sourced Human Primary Cells
We offer an extensive portfolio of human primary cells, including some rare types, isolated from various tissues such as solid tissues, peripheral blood, cord blood, and bone marrow. All primary cells are obtained under the highest ethical and legal standards and are sourced from a wide variety of donors (including young, old, male, female,
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and individuals from different ethnicities), each with detailed donor specifications.
Primary Cells from solid tissues
Following isolation, primary cells from solid tissues are cultured in selective media, and harvested in early passages (P0, P1). PCR testing ensures they are negative for HIV-1, HIV-2, HBV, and HCV. Furthermore, cells are rigorously tested to ensure they are free from mycoplasma, bacterial, or fungal contamination, with thorough monitoring conducted after two weeks of cell culture.
With the capacity to function similarly to in vivo cells, these cells serve as invaluable model systems for drug discovery, toxicity testing, gene therapy, cancer research, and more.
Primary Cells from hematopoietic tissues
We also provide primary cells from (mobilized) peripheral blood, cord blood, and bone marrow, collected and frozen without prior culture. Furthermore, marker-defined subsets, such as hematopoietic stem cells, progenitor cells, and immune cells are available. These subsets are isolated either by positive selection of the wanted cell population or depletion of the unwanted fraction.
Tailored Services for Primary Cells
For long-term projects, specific lots of primary cells can be reserved. Custom Cell Isolation services are available for difficult-to-obtain tissues.
Explore our selective Growth Media optimized for cell culture of human primary cells as well as our extensive portfolio of Extracellular Matrices for both 2D and 3D culture.
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CD14+ Monocytes System Complete Kit
Cat.-Nr: LL-0080
CD14+ Monocytes with RPMI Medium in a Complete Kit. The CD14+ Monocytes System Complete Kit includes: 5M CD14+ cryopreserved Monocytes RPMI 1640-FBS... Read More
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CD19+ B Cells System Complete Kit
Cat.-Nr: LL-0081
Lifeline® CD19+ B Cells System Complete Kit includes: 5 million CD19+ B Cells and RPMI 1640-FBS Cell Culture Medium Complete Kit with Frozen Basal... Read More
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CD4+ T Cells System Complete Kit
Cat.-Nr: LL-0078
Lifeline® Human CD4+ T Cells System Complete Kit includes: Human CD4+ Helper T Cells cryopreserved at 5.000.000 cells per vial (HC-0028) and RPMI... Read More
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CD8+ T Cells System Complete Kit
Cat.-Nr: LL-0079
Lifeline® CD8+ T Cells System Complete Kit includes: 5M CD8+ Cytotoxic T Cells (HC-0029) and RPMI1640-FBS Medium Complete Kit with frozen basal... Read More
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Corneal Epithelial Cells
Cat.-Nr: FC-0029
Lifeline® normal Human Corneal Epithelial Cells (HCEC), when grown in Lifeline® OcuLife™ Medium, provide an ideal serum-free culture model for... Read More
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Dermal Microvascular Endothelial Cells – Adult
Cat.-Nr: FC-0039
Lifeline® normal Human Microvascular Endothelial Cells – Adult, when grown in VascuLife® VEGF-Mv Medium, provide an ideal low-serum culture... Read More
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HAoEC, Human Aortic Endothelial Cells
Cat.-Nr: FC-0014
Lifeline® normal Human Aortic Endothelial Cells, when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture model — with or... Read More
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HAOSMC, Human Aortic Smooth Muscle Cells
Cat.-Nr: FC-0015
Lifeline® smooth muscle cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure... Read More
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HBTEC, Diseased Bronchial/Tracheal Epithelial Cells
Cat.-Nr: FC-0103
Diseased Human Bronchial/Tracheal Epithelial Primary Cells — Cystic Fibrosis. Lifeline® Human Bronchial/Tracheal Epithelial Cells, when grown in... Read More
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HBTEC, Human Bronchial/Tracheal Epithelial Cells P1
Cat.-Nr: FC-0035
Lifeline® Normal Human Bronchial/Tracheal Epithelial Cells (HBTEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free... Read More
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HBTSMC Human Bronchial Tracheal Smooth Muscle Cell
Cat.-Nr: FC-0059
Lifeline® Normal Human Bronchial Tracheal Smooth Muscle Cells (HBTSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum... Read More
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HCAEC, Human Coronary Artery Endothelial Cells
Cat.-Nr: FC-0032
Lifeline® Normal Human Coronary Artery Endothelial Cells (HCAEC), when grown in Lifeline® VascuLife® Medium, provides an ideal culture model, with... Read More
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HCASMC, Human Coronary Artery Smooth Muscle Cells
Cat.-Nr: FC-0031
Lifeline® normal Human Coronary Artery Smooth Muscle Cells (HCASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
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HD-MVECn, Human Dermal MV Endothelial Cells neonatal
Cat.-Nr: FC-0042
Lifeline® Normal Human Dermal Microvascular Endothelial Cells (HD-MVECn), when grown in Lifeline® VascuLife® Medium, provide an ideal culture... Read More
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HDF, Human Dermal Fibroblasts, adult
Cat.-Nr: FC-0024
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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HDF, Human Dermal Fibroblasts, neonatal
Cat.-Nr: FC-0001
Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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HDFn-XF, Human Dermal Fibroblasts neonatal, Xeno-Free
Cat.-Nr: FC-0037
Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal... Read More
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HEM, Human Epidermal Melanocytes, adult
Cat.-Nr: FC-0030
Lifeline® Normal Human Epidermal Melanocytes – Adult (HEMa), when grown in Lifeline® DermaLife Ma Medium, provide an ideal low serum culture... Read More
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HEMa-HP, Human Epidermal Melanocytes - adult, Highly Pigmented
Cat.-Nr: FC-0090
Lifeline® normal Human Epidermal Melanocytes Adult, secondary, highly pigmented (HEMa-HP), when grown in Lifeline® DermaLife Ma Medium, provide an... Read More
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HEMn-HP, Human Epidermal Melanocytes
Cat.-Nr: FC-0089
Lifeline® normal Human Epidermal Melanocytes – Neonatal, Secondary, Highly Pigmented (HEMn-HP), when grown in Lifeline® DermaLife M medium,... Read More
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HEuEC, Human Endometrial Uterine Epithelial Cells
Cat.-Nr: FC-0078
Lifeline® Normal Human Endometrial (Uterine) Epithelial Cells, when grown in Lifeline® ReproLife™ Medium, provide an ideal serum-free culture... Read More
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HIAEC, Human Iliac Artery Endothelial Cells
Cat.-Nr: FC-0028
Lifeline® Iliac Artery Endothelial cells, when grown in Lifeline® VascuLife ® culture medium, provide a low-serum culture ideal for the study of... Read More
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HLSMC, Human Lung Smooth Muscle Cells
Cat.-Nr: FC-0046
Lifeline® normal Human Lung Smooth Muscle Cells, when grown in VascuLife® SMC Medium, provide an ideal culture model for the study of angiogenesis,... Read More
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HMSC-Ad, Human Mesenchymal Stem Cells-Adipose
Cat.-Nr: FC-0034
Lifeline® Normal Human Mesenchymal Stem Cells-Adult (HMSC-Ad) provide an ideal culture model for the study of adult stem cell biology and... Read More
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HMSC-BM, Human Bone Marrow Mesenchymal Stem Cells
Cat.-Nr: FC-0057
Lifeline® Human Bone Marrow Mesenchymal Stem Cells (HMSC-BM) provide an ideal culture model for the study of multipotent stem cell biology and... Read More
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HPAEC, Human Pulmonary Artery Endothelial Cells
Cat.-Nr: FC-0055
Lifeline® normal Human Pulmonary Artery Endothelial Cells (HPAEC), when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture... Read More
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HPASMC, Human Pulmonary Artery Smooth Muscle
Cat.-Nr: FC-0056
Lifeline® normal Human Pulmonary Artery Smooth Muscle Cells (HPASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
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HPrEC, Human Prostate Epithelial Cells
Cat.-Nr: FC-0038
Lifeline® Normal Human Prostate Epithelial Cells (HPrEC), when grown in ProstaLifeTM Medium, provide an ideal serum-free and retinoic acid-free... Read More
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HSAEC, Diseased Human Small Airway Epithelial Cells
Cat.-Nr: FC-0067
Diseased Human Small Airway Epithelial Primary Cells — Asthma + COPD (Chronic Obstructive Pulmonary Disease).These primary cells have been... Read More
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HSAEC, Human Small Airway Epithelial Cells, P1
Cat.-Nr: FC-0016
Lifeline® normal Human Small Airway Epithelial Cells (HSAEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free culture... Read More
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HSkMC Human Skeletal Muscle Satellite Cells
Cat.-Nr: FC-0091
Lifeline® Human Skeletal Muscle Satellite Cells (HSkMC) provide an ideal culture model for the study of muscle cell biology, diabetes, muscle cell... Read More
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Human Bladder Apex Epithelial Cells
Cat.-Nr: FC-0040
Lifeline® Normal Human Bladder Apex Epithelial Cells, when grown in UroLife™ Bladder Epithelial Medium Complete Kit, provide an ideal culture... Read More
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Human Bladder Dome Epithelial Cells
Cat.-Nr: FC-0079
Lifeline® Primary Normal Human Bladder Dome Epithelial Cells, when grown in UroLifeTM Culture Medium Complete Kit, provide an ideal culture model... Read More
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Human Bladder Fibroblasts
Cat.-Nr: FC-0050
Lifeline® normal Human Bladder Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
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Human Bladder Smooth Muscle Cells, Primary (HBSMC)
Cat.-Nr: FC-0043
Lifeline® Normal Human Bladder Smooth Muscle Cells (HBSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 0.5M
Cat.-Nr: ASEBMCD34-C0.5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 1M
Cat.-Nr: ASEBMCD34-C1M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 5M
Cat.-Nr: ASEBMCD34-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 10M
Cat.-Nr: ASEBMMN-C10M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 20M
Cat.-Nr: ASEBMMN-C20M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 50M
Cat.-Nr: ASEBMMN-C50M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 5M
Cat.-Nr: ASEBMMN-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cardiac Microvascular Endothelial Cells
Cat.-Nr: FC-0053
Lifeline® Normal Human Cardiac Microvascular Endothelial Cells, when grown in Lifeline® VascuLife® VEGF-Mv Medium, provide an ideal low-serum... Read More
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Human CD14+ Monocytes from Leukapheresed Peripheral Blood 5M
Cat.-Nr: HC-0030
Human CD14+ Monocytes provide an ideal culture model for the study of immunology. These human CD14+ Monocytes are purified... Read More
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Human CD19+ B Cells
Cat.-Nr: HC-0031
Lifeline® Human CD19+ B Cells provide an ideal culture model for the study of immunology. These normal human CD19+ B Cells are purified from... Read More
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Human CD3+ Pan T 10M System Complete Kit
Cat.-Nr: LL-0077
The CD3+ Pan T Cell 10M System Complete Kit includes: Human CD3+ Pan T Cells with 10 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
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Human CD3+ Pan T 5 M Cells
Cat.-Nr: HC-0026
Human CD3+ Pan T Cells provide an ideal culture model for the study of immunology, immunotherapy, autoimmunity, cancer, cell-based therapies, and... Read More
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Human CD3+ Pan T 5M System Complete Kit
Cat.-Nr: LL-0076
The CD3+ Pan T Cell 5M System Complete Kit includes: Human CD3+ Pan T Cells with 5 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
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Human CD3+ Pan T Cells 10 M
Cat.-Nr: HC-0027
Human CD3+ Pan T Cells are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability. Human CD3+ Pan T Cells are... Read More
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Human CD34-depleted Bone Marrow Mononuclear Cells - Single Donor 100M
Cat.-Nr: ASEBMCD34-deplete
Bone Marrow Mononuclear cells (BMMCs) undergo immunomagnetic isolation to deplete CD34+ cells. This preparation encompasses non-CD34+ cells,... Read More