Primary Cells
Comprehensive Collection of Ethically Sourced Human Primary Cells
We offer an extensive portfolio of human primary cells, including some rare types, isolated from various tissues such as solid tissues, peripheral blood, cord blood, and bone marrow. All primary cells are obtained under the highest ethical and legal standards and are sourced from a wide variety of donors (including young, old, male, female, and individuals from different ethnicities), each with detailed donor specifications.
Primary Cells from solid tissues
Following isolation, primary cells from solid tissues are cultured in selective media, and harvested in early passages (P0, P1). PCR testing ensures they are negative for HIV-1, HIV-2, HBV, and HCV. Furthermore, cells are rigorously tested to ensure they are free from mycoplasma, bacterial, or fungal contamination, with thorough monitoring conducted after two weeks of cell culture.
With the capacity to function similarly to in vivo cells, these cells serve as invaluable model systems for drug discovery, toxicity testing, gene therapy, cancer research, and more.
Primary Cells from hematopoietic tissues
We also provide primary cells from (mobilized) peripheral blood, cord blood, and bone marrow, collected and frozen without prior culture. Furthermore, marker-defined subsets, such as hematopoietic stem cells, progenitor cells, and immune cells are available. These subsets are isolated either by positive selection of the wanted cell population or depletion of the unwanted fraction.
Tailored Services for Primary Cells
For long-term projects, specific lots of primary cells can be reserved. Custom Cell Isolation services are available for difficult-to-obtain tissues.
Explore our selective Growth Media optimized for cell culture of human primary cells as well as our extensive portfolio of Extracellular Matrices for both 2D and 3D culture.
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Human Oral Keratinocytes - Gingiva
Cat.-Nr: FC-0094
Lifeline® normal Human Oral Keratinocytes (Gingiva), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for the... Read More
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CD14+ Monocytes System Complete Kit
Cat.-Nr: LL-0080
CD14+ Monocytes with RPMI Medium in a Complete Kit. The CD14+ Monocytes System Complete Kit includes: 5M CD14+ cryopreserved Monocytes RPMI 1640-FBS... Read More
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CD19+ B Cells System Complete Kit
Cat.-Nr: LL-0081
Lifeline® CD19+ B Cells System Complete Kit includes: 5 million CD19+ B Cells and RPMI 1640-FBS Cell Culture Medium Complete Kit with Frozen Basal... Read More
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CD4+ T Cells System Complete Kit
Cat.-Nr: LL-0078
Lifeline® Human CD4+ T Cells System Complete Kit includes: Human CD4+ Helper T Cells cryopreserved at 5.000.000 cells per vial (HC-0028) and RPMI... Read More
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CD8+ T Cells System Complete Kit
Cat.-Nr: LL-0079
Lifeline® CD8+ T Cells System Complete Kit includes: 5M CD8+ Cytotoxic T Cells (HC-0029) and RPMI1640-FBS Medium Complete Kit with frozen basal... Read More
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Diseased Human Small Airway Epithelial Cells
Cat.-Nr: FC-0067
Diseased Human Small Airway Epithelial Primary Cells — Asthma + COPD (Chronic Obstructive Pulmonary Disease).These primary cells have been... Read More
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Highly Pigmented Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0090
Lifeline® normal Human Epidermal Melanocytes Adult, secondary, highly pigmented (HEMa-HP), when grown in Lifeline® DermaLife Ma Medium, provide an... Read More
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Highly Pigmented Human Neonatal Epidermal Melanocytes
Cat.-Nr: FC-0089
Lifeline® normal Human Epidermal Melanocytes – Neonatal, Secondary, Highly Pigmented (HEMn-HP), when grown in Lifeline® DermaLife M medium,... Read More
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Human Adult Dermal Fibroblasts - HDFad
Cat.-Nr: FC-0024
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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Human Adult Epidermal Keratinocytes - NHEKad
Cat.-Nr: FC-0025
Lifeline® Normal Human Epidermal Keratinocytes adult (HEKa), when grown in Lifeline® DermaLife K Medium, provides an ideal serum-free culture... Read More
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Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0030
Lifeline® Normal Human Epidermal Melanocytes – Adult (HEMa), when grown in Lifeline® DermaLife Ma Medium, provide an ideal low serum culture... Read More
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Human Aortic Endothelial Cells
Cat.-Nr: FC-0014
Lifeline® normal Human Aortic Endothelial Cells, when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture model — with or... Read More
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Human Aortic Smooth Muscle Cells
Cat.-Nr: FC-0015
Lifeline® smooth muscle cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure... Read More
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Human Bladder Apex Epithelial Cells
Cat.-Nr: FC-0040
Lifeline® Normal Human Bladder Apex Epithelial Cells, when grown in UroLife™ Bladder Epithelial Medium Complete Kit, provide an ideal culture... Read More
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Human Bladder Dome Epithelial Cells
Cat.-Nr: FC-0079
Lifeline® Primary Normal Human Bladder Dome Epithelial Cells, when grown in UroLifeTM Culture Medium Complete Kit, provide an ideal culture model... Read More
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Human Bladder Fibroblasts
Cat.-Nr: FC-0050
Lifeline® normal Human Bladder Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
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Human Bladder Smooth Muscle Cells
Cat.-Nr: FC-0043
Lifeline® Normal Human Bladder Smooth Muscle Cells (HBSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 0.5M
Cat.-Nr: ASEBMCD34-C0.5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 1M
Cat.-Nr: ASEBMCD34-C1M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 5M
Cat.-Nr: ASEBMCD34-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mesenchymal Stem Cells - MSC-BM
Cat.-Nr: FC-0057
Lifeline® Human Bone Marrow Mesenchymal Stem Cells (HMSC-BM) provide an ideal culture model for the study of multipotent stem cell biology and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 10M
Cat.-Nr: ASEBMMN-C10M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 20M
Cat.-Nr: ASEBMMN-C20M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 50M
Cat.-Nr: ASEBMMN-C50M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 5M
Cat.-Nr: ASEBMMN-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bronchial Tracheal Smooth Muscle Cells
Cat.-Nr: FC-0059
Lifeline® Normal Human Bronchial Tracheal Smooth Muscle Cells (HBTSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum... Read More
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Human Cardiac Fibroblasts
Cat.-Nr: FC-0060
Lifeline® Normal Human Cardiac Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell... Read More
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Human Cardiac Microvascular Endothelial Cells
Cat.-Nr: FC-0053
Lifeline® Normal Human Cardiac Microvascular Endothelial Cells, when grown in Lifeline® VascuLife® VEGF-Mv Medium, provide an ideal low-serum... Read More
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Human CD14+ Monocytes from Leukapheresed Peripheral Blood 5M
Cat.-Nr: HC-0030
Human CD14+ Monocytes provide an ideal culture model for the study of immunology. These human CD14+ Monocytes are purified... Read More
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Human CD19+ B Cells
Cat.-Nr: HC-0031
Lifeline® Human CD19+ B Cells provide an ideal culture model for the study of immunology. These normal human CD19+ B Cells are purified from... Read More
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Human CD3+ Pan T 10M System Complete Kit
Cat.-Nr: LL-0077
The CD3+ Pan T Cell 10M System Complete Kit includes: Human CD3+ Pan T Cells with 10 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
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Human CD3+ Pan T 5M Cells
Cat.-Nr: HC-0026
Human CD3+ Pan T Cells provide an ideal culture model for the study of immunology, immunotherapy, autoimmunity, cancer, cell-based therapies, and... Read More
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Human CD3+ Pan T 5M System Complete Kit
Cat.-Nr: LL-0076
The CD3+ Pan T Cell 5M System Complete Kit includes: Human CD3+ Pan T Cells with 5 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
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Human CD3+ Pan T Cells 10M
Cat.-Nr: HC-0027
Human CD3+ Pan T Cells are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability. Human CD3+ Pan T Cells are... Read More
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Human CD34-depleted Bone Marrow Mononuclear Cells - Single Donor 100M
Cat.-Nr: ASEBMCD34-deplete
Bone Marrow Mononuclear cells (BMMCs) undergo immunomagnetic isolation to deplete CD34+ cells. This preparation encompasses non-CD34+ cells,... Read More
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Human CD34+ Cord Blood Stem / Progenitor Cells (Single Donor) 1M
Cat.-Nr: ASECBCD34-C1M
Human Cord Blood CD34+ Stem / Progenitor Cells Frozen, single Donor If you’d like to receive more information about this product, please send us... Read More
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Human CD4+ T Cells
Cat.-Nr: HC-0028
Lifeline® Human CD4+ T Cells provide an ideal culture model for the study of immunology. These human CD4+ T Cells are purified from leukapharesed... Read More
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Human CD8+ T Cells
Cat.-Nr: HC-0029
Lifeline® Human CD8+ T Cells provide an ideal culture model for the study of immunology. Human CD8+ T Cells are purified from leukapharesed... Read More
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Human Cervical Epithelial Cells
Cat.-Nr: FC-0080
Lifeline® Normal Human Cervical Epithelial Cells (HCxEC), when grown in Lifeline® ReproLife™ CX Medium, provide an ideal serum-free culture model... Read More
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Human Cord Blood CD14+ Monocytes 5M
Cat.-Nr: ASECBCD14-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD19+ B Cells 2.5M
Cat.-Nr: ASECBCD19-C2.5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD19+ B Cells 5M
Cat.-Nr: ASECBCD19-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD3+ Pan T Cells 10M
Cat.-Nr: ASECBCD3-C10M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD3+ Pan T Cells 20M
Cat.-Nr: ASECBCD3-C20M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD34+ Stem / Progenitor Cells (Single Donor) 0.5M
Cat.-Nr: ASECBCD34-C0.5M
CD34+ stem cells are multipotent cells that can be found in human umbilical cord blood. These cells have the capacity to differentiate to all blood... Read More
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Human Cord Blood CD34+ Stem & Progenitor Cells (Mixed Donor) 0.5M
Cat.-Nr: ASECBCD34mix-C0.5M
CD34+ stem cells are multipotent cells that can be found in human umbilical cord blood. These cells have the capacity to differentiate to all blood... Read More
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Human Cord Blood CD34+ Stem & Progenitor Cells (Mixed Donor) 1M
Cat.-Nr: ASECBCD34mix-C1M
CD34+ stem cells are multipotent cells that can be found in human umbilical cord blood. These cells have the capacity to differentiate to all blood... Read More
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Human Cord Blood CD34+ Stem & Progenitor Cells (Mixed Donor) 5M
Cat.-Nr: ASECBCD34mix-C5M
CD34+ stem cells are multipotent cells that can be found in human umbilical cord blood. These cells have the capacity to differentiate to all blood... Read More
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Human Cord Blood CD4+/ CD45RA+ Naive T Cells 10M
Cat.-Nr: ASECBCD4-45RA-C10M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Cord Blood CD4+/ CD45RA+ Naive T Cells 15M
Cat.-Nr: ASECBCD4-45RA-C15M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More