- Species: Human
- Cell Type: Melanocytes
- Tissue: Skin
- Additional Attributes: Primary Cells
- Specific Attributes: Neonatal
- Features:Single donor, cryopreserved as secondary cells
Lifeline® normal Human Epidermal Melanocytes – Neonatal, Secondary, Highly Pigmented (HEMn-HP), when grown in Lifeline® DermaLife M medium, provide an ideal low serum culture model, without PMA or Cholera toxin, for the accurate testing of compounds and for use in the study of cancer or dermal biology.
Lifeline® Human Epidermal Melanocytes Neonatal have been isolated, plated, and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest viability and plating efficiency.
Our HEMn-HP are quality tested in DermaLife M Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
Lifeline® HEM are not exposed to antimicrobials or phenol red when cultured in Lifeline® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements; however they are not needed, or recommended, to achieve optimal cell performance in most research applications.
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™