Fluorogenic Substrates & Inhibitors
Fluorogenic Substrates for Coagulation Enzymes & Inhibitors
Fluorogenic substrates and inhibitors are commonly used in haemostasis research to study various aspects of the coagulation cascade and thrombus formation.
Our products include a complete line of fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN)-based substrates designed for monitoring the enzyme activity of serine proteases, as well as tripeptide chloromethylketones that irreversibly inhibit these proteases. Additionally, we offer a variety of other inhibitors, both plasma and non-plasma based.
…read more
ANSN-Based Fluorogenic Substrates: These are synthetic substrates that are designed to emit fluorescence when cleaved by specific enzymes involved in the coagulation cascade. By measuring the fluorescence emitted, researchers can quantify the activity of these enzymes in real-time. For example, fluorogenic substrates can be used to measure the activity of Factor Xa, Thrombin, Factor XIa, activated Protein C (aPC), Plasmin, tissue Plasminogen Activator (tPA) and Factor VIIa, or other proteases involved in coagulation.
Inhibitors: Inhibitors are compounds that block the activity of specific enzymes or proteins involved in coagulation. In haemostasis research, inhibitors are often used to study the effects of blocking certain steps in the coagulation cascade. For example, inhibitors of Thrombin or Factor Xa can be used to investigate their roles in clot formation and stability.
Explore a wide range of inhibitors, from human protease inhibitors like Angiostatin and Thrombin Activatable Fibrinolysis Inhibitor (TAFI) to various Chloromethylketones. Furthermore, you can choose from Corn Trypsin Inhibitor (CTI) and the synthetic Thrombin inhibitor Dansylarginine N-(3-ethyl-1,5-pentanediyl)amide (DAPA) for your research needs.
Notably, CTI is available pre-aliquoted in our blood collection tubes, specifically our SCAT-27 line. These CTI Vacuum Collection Tubes (SCAT-Type 27) are for research use only and streamline Tissue Factor-dependent studies by enabling direct blood collection onto an anticoagulant (Sodium Citrate) containing CTI.
Fluorogenic substrates and inhibitors provide valuable tools monitoring haemostasis and thrombosis, as well as for developing novel diagnostic and therapeutic strategies for related disorders.
-
Biotinylated EGR-chloromethylketone
Cat.-Nr: BEGRCK-06
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
Biotinylated FPR-chloromethylketone
Cat.-Nr: BFPRCK-06
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
BOC-D-VLR-ANSNH
Cat.-Nr: SN-54
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
BOC-L-(p-F)FPR-ANSNH
Cat.-Nr: SN-18
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
BOC-L-FPR-ANSNH
Cat.-Nr: SN-20
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
Corn Trypsin Inhibitor
Cat.-Nr: CTI-01
Corn trypsin inhibitor (CTI) is a small protein that is localized in the kernels of most species of corn. CTI is not only an inhibitor of trypsin,... Read More
-
D-AFK-ANSNH
Cat.-Nr: SN-5
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
D-FPR-ANSNH-C4H9(2HCl)
Cat.-Nr: SN-17c
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
D-FPR-ANSNH-C6H11X(2HCI)
Cat.-Nr: SN-17a
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
D-LPR-ANSNH-C3H7
Cat.-Nr: SN-13a
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
D-VPR-ANSNH
Cat.-Nr: SN-59
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
Dansylarginine-N-(3-ethyl-1,5-pentanediyl)amide (DAPA)
Cat.-Nr: DAPA
Dansylarginine N-(3-ethyl-1,5-pentanediyl)amide, more commonly referred to as DAPA, is a potent (Ki=10-7M) and specific synthetic inhibitor of... Read More
-
EGR-chloromethylketone (GGACK)
Cat.-Nr: EGRCK-01
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
Fluorescein EGR-chloromethylketone
Cat.-Nr: FEGRCK-06
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
Fluorescein FPR-chloromethylketone
Cat.-Nr: FFPRCK-06
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
FPR-chloromethylketone (PPACK)
Cat.-Nr: FPRCK-01
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common... Read More
-
Human Alpha-2-Plasmin Inhibitor
Cat.-Nr: HA2AP-0230
α2 –plasmin inhibitor (α2 -PI) is a single-chain glycoprotein and is one of the major serine proteinase inhibitors circulating in plasma.... Read More
-
Human Angiostatin
Cat.-Nr: ANG-01
Angiostatin is a single-chain, proteolytic fragment of glu-plasminogen which has a molecular weight of about 38,000. It is a potent inhibitor of... Read More
-
Human Antithrombin III
Cat.-Nr: HCATIII-0120
Antithrombin III (ATIII) is a single chain glycoprotein with a molecular weight of 58,000. It is a member of the serpin (serine protease inhibitor)... Read More
-
Human Heparin Cofactor II
Cat.-Nr: HCII-0190
Heparin cofactor II (HCII) (heparin cofactor A, antithrombin BM, dermatan sulfate cofactor, human leuserpin-2) is a single chain glycoprotein (Mr =... Read More
-
Human Thrombin-Activatable Fibrinolysis Inhibitor (TAFI)
Cat.-Nr: TAFI-01
Thrombin Activatable Fibrinolysis Inhibitor (TAFI, Plasma pro-carboxypeptidase B, carboxypeptidase U) is a single chain glycoprotein zymogen (Mr =... Read More
-
L-EGR-ANSNH-C3H7
Cat.-Nr: SN-45
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
MES-D-LGR-ANSN
Cat.-Nr: SN-7
Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity... Read More
-
Mouse Antithrombin III
Cat.-Nr: MCATIII-5120
Antithrombin III (ATIII) is a single chain glycoprotein with a molecular weight of 58,000. It is a member of the serpin (serine protease inhibitor)... Read More