We are here to help   +49 (0) 2241.255 15 0
  • 0Shopping Cart
  • Product Types
      • Coagulation Proteins
      • Enzymes
      • Cells and Media
      • Cytokines and Chemokines
      • COVID-19 / SARS-CoV-2
      • Molecular Biology
      • Stem Cells / iPSC
      • Adhesion Molecules
      • Tissue Dissociation
      • Matrix Proteins / Bioprinting
      • Gene Editing / CRISPR / TARGATT
  • Brands
  • Company
  • Services
  • Career
  • News
  • Contact
  • Menu Menu
Home |Products|Coagulation Proteins|dansylarginine-N-(3-ethyl-1,5-pentanediyl)amide

dansylarginine-N-(3-ethyl-1,5-pentanediyl)amide

Cat.-Nr.: DAPA

Description

Dansylarginine N-(3-ethyl-1,5-pentanediyl)amide, more commonly referred to as DAPA, is a potent (Ki=10-7M) and specific synthetic inhibitor of thrombin (1). Of special interest are the unique fluorescent properties contributed by the dansyl moiety of DAPA. When bound to thrombin, the fluorescence intensity and lifetime of the dansyl moiety are increased three fold. The enhancement of fluorescence intensity, coupled with its inhibitory properties, have made this compound extremely useful for studies involving thrombin generation (2-7). In addition to an increase in fluorescence intensity and lifetime, there is a decrease in depolarization of the excitation signal which also supports fluorescence polarization studies. The inhibitor properties of DAPA alone have been useful in a variety of applications. DAPA is routinely used as a protease inhibitor during the isolation of proteins that are susceptible to thrombin cleavage. Furthermore, by eliminating the feedback of thrombin activity during prothrombin activation, the isolation of reaction intermediates such as fragment 1•2, and meizothrombin have been made possible (7). DAPA is synthesized according the procedure described by Nesheim, et al. (1). HPLC, TLC and spectral analyses are used to determine the purity of the final product. DAPA is supplied in water. When properly stored at -20°C and protected from light, the compound is stable for many years.

  • SUPPLIER:

    Haematologic TechnologiesProlytix

  • STATUS:

    In Stock

  • SIZE:

    1 mg

  • Overview

Overview

  • Species: Synthetic
  • Additional Attributes: Inhibitors
  • Formulation:H2O
  • Localization:Synthetic
  • Mode of action:Protease Inhibitor
  • Structure:C25H39O3N6S1Cl1

RELATED PRODUCTS


  • Anti-Human Factor XII

  • Bovine Factor Va

  • Corn Trypsin Inhibitor

  • Human Factor X

  • Imprint
  • Privacy policy
DermaLife K, Keratinocyte Growth Medium ChondroLife Complete Chondrogenesis Medium
Scroll to top