- Species: Human
- Cell Type: Fibroblast
- Tissue: Skin
- Additional Attributes: Primary Cells
- Specific Attributes: Neonatal
- Features:Single donor, from human foreskin, cryopreserved as primary cells
Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Lifeline® Dermal Fibroblasts – Neonatal are isolated from neonatal human foreskin and cryopreserved as primary cells to ensure the highest viability and plating efficiency.
Our skin fibroblasts are quality tested in FibroLife® S2 or FibroLife Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
Lifeline® dermal fibroblasts tested in both FibroLife® Serum-Free Medium and FibroLife® S2 Medium display optimal growth at rates equal to or greater than classical serum-supplemented medium.
500,000 cells per vial
Lifeline® dermal fibroblasts – neonatal are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Dermal fibroblasts are quality tested for:
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
For an overview of available human primary fibroblasts from different tissues and recommended culture media (click).
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™