- Species: Human
- Cell Type: Fibroblasts
- Tissue: Skin
- Additional Attributes: Primary Cells
- Specific Attributes: Adult
- Features:Single donor, from human foreskin, cryopreserved as primary cells
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Lifeline® Dermal Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency.
Our fibroblasts are quality tested in both FibroLife® Serum-Free Medium and FibroLife® S2 Medium to ensure:
Optimal growth at rates equal to or greater than classical serum-supplemented medium
Adult Dermal Fibroblasts are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline® FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Dermal fibroblasts are quality tested for:
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
For an overview of available human primary fibroblasts from different tissues and recommended culture media (click).
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™