- Species: Human
- Cell Type: Fibroblast
- Tissue: Skin
- Additional Attributes: Primary Cells
- Specific Attributes: Neonatal
- Features:Single donor, from human foreskin, cryopreserved as primary cells, Xeno-Free
Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human pluripotent stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
HDFn-XF are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HDFn-XF are quality tested in FibroLife® Xeno-Free Complete Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media containing non-human proteins or supplements.
Lifeline® HDFn-XF are not exposed to antimicrobials or phenol red when cultured in FibroLife® Xeno-Free Complete Medium, an advantage since these supplements can cause cell stress and ” masking effects” that may negatively impact experimental results.
500,000 cells per vial
Neonatal dermal fibroblasts are tested for:
Proliferation and morphology: Normal cell appearance for 15 population doublings
Cell viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma. Negative for bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Lifeline® documents that all materials used in the manufacture of products which are labeled “Xeno-Free” have never come into contact with material of animal (non-human) origin and are not of animal (non-human) origin.
Lifeline Cell Technology™