- Species: Human
- Cell Type: Endothelial Cells, Microvascular Endothelial Cells
- Tissue: Skin
- Additional Attributes: Primary Cells
- Features:Single donor, Cryopreserved after less than 4 weeks in culture, von Willebrand Factor positive
Lifeline® Normal Human Dermal Microvascular Endothelial Cells (HD-MVECn), when grown in Lifeline® VascuLife® Medium, provide an ideal culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis, or vascular biology.
Lifeline® HDMVECn are isolated and quality tested in VascuLife® EnGS-Mv Medium to ensure optimal growth over a period of at least 15 population doublings while remaining naïve to VEGF.
Lifeline® HDMVECn need not be exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and ” masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements, however they are not needed or recommended to achieve optimal cell performance in most research applications.
HDMVECn are cryopreserved after less than 4 weeks in culture
Culture HDMVECn with or without VEGF.
Lifeline guarantees performance and quality
500,000 cells per vial
HDMVECn are extensively tested for quality and optimal performance:
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma. Negative for bacterial and fungal growth.
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Specific Staining: von Willebrand Factor
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™