- Species: Human
- Cell Type: Epithelial
- Tissue: Lung / Airway
- Additional Attributes: Primary Cells
- Features:Single donor, from human lung tissue, cryopreserved as primary cells
Lifeline® normal Human Small Airway Epithelial Cells (HSAEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free culture model for the study of respiratory infection, asthma, chronic obstructive pulmonary disease, cystic fibrosis, response to inflammation, and the damaging effects of smoking.
Airway epithelial cells are cryopreserved after primary culture, e.g., cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Lifeline® small airway epithelial cells are not exposed to retinoic acid during isolation or expansion.
Small Airway Epithelial Cells are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HSAEC are extensively tested in BronchiaLife™ Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Lifeline® guarantees performance and quality.
HSAEC are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline medium.
HSAEC are tested for:
Proliferation and Morphology: Normal cell appearance for 15 population doublings
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma, bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
500,000 cryopreserved cells per vial
Lifeline Cell Technology™