- Species: Human
- Cell Type: Epithelial
- Tissue: Eye
- Additional Attributes: Primary Cells
- Features:Single donor, from human corneal tissue, cryopreserved as secondary cells
Lifeline® normal Human Corneal Epithelial Cells (HCEC), when grown in Lifeline® OcuLife™ Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HCEC are cell-matrix interactions, gene regulation and tissue development, drug development, and validation of alternative methods in toxicology.
HCEC are isolated from human corneal tissue and expanded twice in culture vessels before being harvested for cryopreservation.Our HCEC are quality tested in OcuLife™ Medium to ensure optimal morphology and growth over a period of at least three passages.Lifeline® HCEC are not exposed to antimicrobials or phenol red when cultured in OcuLife™ Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements, but they aren’t recommended or needed to achieve optimal cell performance in most research applications.
Human Corneal Epithelial Cells are tested for:
Cell Count: 500,000 cryopreserved cells per vial
Proliferation and Morphology in Fully Supplemented OcuLife™ Medium: Normal cell appearance for 3 passages
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma, Negative for bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
500,000 cells per vial
Lifeline Cell Technology™