- Species: Human
- Cell Type: Endothelial
- Tissue: Illiac
- Additional Attributes: Primary Cells
- Features:Single donor, from human illiac artery, cryopreserved as secondary cells
Lifeline® Iliac Artery Endothelial cells, when grown in Lifeline® VascuLife ® culture medium, provide a low-serum culture ideal for the study of atherosclerosis and other cardiovascular diseases, general vascular biology research, wound healing and cell migration studies.
Human Iliac Artery Endothelial Cells are isolated in a specialized endothelial cell medium called VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF. This specialized medium provides a low-serum (2%) environment with or without VEGF that results in optimal endothelial cell growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
Lifeline® iliac artery endothelial cells are plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest viability, purity, and plating efficiency.
These cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium.
Iliac atery endothelial cells are quality tested for:
Proliferation and Morphology: Normal cell appearance for 15 population doublings
Cell Viability: Minimum 70% viability when thawed from cryopreservation
Sterility Testing: Negative for mycoplasma, negative for bacterial and fungal growth
Virus Testing: Negative for HIV-1, HIV-2, HBV and HCV by PCR
Specific Staining: von Willebrand factor positive. Smooth muscle a-actin negative.
500,000 cells per vial
Lifeline Cell Technology™