- Species: Human
- Additional Attributes: Primary Cells
- Tissue: Blood
- Cell Type: Hematopoietic cells
- Source: Peripheral Blood, leukapheresed
Human CD14+ Monocytes provide an ideal culture model for the study of immunology. These human CD14+ Monocytes are purified from leukapharesed peripheral blood using immunomagnetic positive selection.
Normal CD14+ Monocytes are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability and plating efficiency. Our Human CD14+ monocytes are quality tested via flow cytometry to ensure the enrichment of CD14+ cells. 5M cells per vial.
CD14+ Monocytes are maintained in a suspension culture. Monocytes will not proliferate under maintenance conditions.
CD14+ Monocytes are not exposed to antimicrobials or phenol red.
Use RPMI1640-FBS Medium Complete Kit with CD14+ Monocytes. The Kit includes frozen basal medium containing phenol red, 2 mM L-glutamine, 25 mM HEPES and 1 mM sodium Pyruvate. The Complete Kit also includes Fetal Bovine Serum Gentamicin and Amphotericin B.
For an overview of available hematopoietic cells and isolated subsets from all available sources (leukapheresis, periperal blood, cord blood, bone marrow and iPSC-derived), see the product list: Human Hematopoietic Cells (click).
Lifeline Cell Technology™