Primary Cells
Comprehensive Collection of Ethically Sourced Human Primary Cells
We offer an extensive portfolio of human primary cells, including some rare types, isolated from various tissues such as solid tissues, peripheral blood, cord blood, and bone marrow. All primary cells are obtained under the highest ethical and legal standards and are sourced from a wide variety of donors (including young, old, male, female, and individuals from different ethnicities), each with detailed donor specifications.
Primary Cells from solid tissues
Following isolation, primary cells from solid tissues are cultured in selective media, and harvested in early passages (P0, P1). PCR testing ensures they are negative for HIV-1, HIV-2, HBV, and HCV. Furthermore, cells are rigorously tested to ensure they are free from mycoplasma, bacterial, or fungal contamination, with thorough monitoring conducted after two weeks of cell culture.
With the capacity to function similarly to in vivo cells, these cells serve as invaluable model systems for drug discovery, toxicity testing, gene therapy, cancer research, and more.
Primary Cells from hematopoietic tissues
We also provide primary cells from (mobilized) peripheral blood, cord blood, and bone marrow, collected and frozen without prior culture. Furthermore, marker-defined subsets, such as hematopoietic stem cells, progenitor cells, and immune cells are available. These subsets are isolated either by positive selection of the wanted cell population or depletion of the unwanted fraction.
Tailored Services for Primary Cells
For long-term projects, specific lots of primary cells can be reserved. Custom Cell Isolation services are available for difficult-to-obtain tissues.
Explore our selective Growth Media optimized for cell culture of human primary cells as well as our extensive portfolio of Extracellular Matrices for both 2D and 3D culture.
-
Human Oral Keratinocytes - Gingiva
Cat.-Nr: FC-0094
Lifeline® normal Human Oral Keratinocytes (Gingiva), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for the... Read More
-
CD14+ Monocytes - Complete Kit
Cat.-Nr: LL-0080
CD14+ Monocytes with RPMI Medium in a Complete Kit. The CD14+ Monocytes System Complete Kit includes: 5M CD14+ cryopreserved Monocytes RPMI 1640-FBS... Read More
-
CD19+ B-Cells - Complete Kit
Cat.-Nr: LL-0081
Lifeline® CD19+ B Cells System Complete Kit includes: 5 million CD19+ B Cells and RPMI 1640-FBS Cell Culture Medium Complete Kit with Frozen Basal... Read More
-
CD4+ T-Cells - Complete Kit
Cat.-Nr: LL-0078
Lifeline® Human CD4+ T Cells System Complete Kit includes: Human CD4+ Helper T Cells cryopreserved at 5.000.000 cells per vial (HC-0028) and RPMI... Read More
-
CD8+ T-Cells - Complete Kit
Cat.-Nr: LL-0079
Lifeline® CD8+ T Cells System Complete Kit includes: 5M CD8+ Cytotoxic T Cells (HC-0029) and RPMI1640-FBS Medium Complete Kit with frozen basal... Read More
-
Diseased Human Bronchial/Tracheal Epithelial Cells
Cat.-Nr: FC-0103
Diseased Human Bronchial/Tracheal Epithelial Primary Cells — Cystic Fibrosis. Lifeline® Human Bronchial/Tracheal Epithelial Cells, when grown in... Read More
-
Diseased Human Small Airway Epithelial Cells
Cat.-Nr: FC-0067
Diseased Human Small Airway Epithelial Primary Cells — Asthma + COPD (Chronic Obstructive Pulmonary Disease). These primary cells have been... Read More
-
Highly Pigmented Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0090
Lifeline® normal Human Epidermal Melanocytes Adult, secondary, highly pigmented (HEMa-HP), when grown in Lifeline® DermaLife Ma Medium, provide an... Read More
-
Highly Pigmented Human Neonatal Epidermal Melanocytes
Cat.-Nr: FC-0089
Lifeline® normal Human Epidermal Melanocytes – Neonatal, Secondary, Highly Pigmented (HEMn-HP), when grown in Lifeline® DermaLife M medium,... Read More
-
Human Adult Dermal Fibroblasts - HDFad
Cat.-Nr: FC-0024
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human... Read More
-
Human Adult Epidermal Keratinocytes - NHEKad
Cat.-Nr: FC-0025
Lifeline® Normal Human Epidermal Keratinocytes adult (HEKa), when grown in Lifeline® DermaLife K Medium, provides an ideal serum-free culture... Read More
-
Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0030
Lifeline® Normal Human Epidermal Melanocytes – Adult (HEMa), when grown in Lifeline® DermaLife Ma Medium, provide an ideal low serum culture... Read More
-
Human Aortic Endothelial Cells
Cat.-Nr: FC-0014
Lifeline® normal Human Aortic Endothelial Cells (HAECs), when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture model —... Read More
-
Human Aortic Smooth Muscle Cells
Cat.-Nr: FC-0015
Lifeline® smooth muscle cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure... Read More
-
Human Bladder Apex Epithelial Cells
Cat.-Nr: FC-0040
Lifeline® Normal Human Bladder Apex Epithelial Cells, when grown in UroLife™ Bladder Epithelial Medium Complete Kit, provide an ideal culture... Read More
-
Human Bladder Dome Epithelial Cells
Cat.-Nr: FC-0079
Lifeline® Primary Normal Human Bladder Dome Epithelial Cells, when grown in UroLifeTM Culture Medium Complete Kit, provide an ideal culture model... Read More
-
Human Bladder Fibroblasts
Cat.-Nr: FC-0050
Lifeline® normal Human Bladder Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
-
Human Bladder Smooth Muscle Cells
Cat.-Nr: FC-0043
Lifeline® Normal Human Bladder Smooth Muscle Cells (HBSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
-
Human Bone Marrow Mesenchymal Stem Cells - MSC-BM
Cat.-Nr: FC-0057
Lifeline® Human Bone Marrow Mesenchymal Stem Cells (HMSC-BM) provide an ideal culture model for the study of multipotent stem cell biology and... Read More
-
Human Bronchial Tracheal Smooth Muscle Cells
Cat.-Nr: FC-0059
Lifeline® Normal Human Bronchial Tracheal Smooth Muscle Cells (HBTSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum... Read More
-
Human Bronchial/Tracheal Epithelial Cells P0
Cat.-Nr: FC-0035
Lifeline® Normal Human Bronchial/Tracheal Epithelial Cells (HBTEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free... Read More
-
Human Cardiac Fibroblasts
Cat.-Nr: FC-0060
Lifeline® Normal Human Cardiac Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell... Read More
-
Human Cardiac Microvascular Endothelial Cells
Cat.-Nr: FC-0053
Lifeline® Normal Human Cardiac Microvascular Endothelial Cells, when grown in Lifeline® VascuLife® VEGF-Mv Medium, provide an ideal low-serum... Read More
-
Human CD14+ Monocytes from Leukapheresed Peripheral Blood
Cat.-Nr: HC-0030
Human CD14+ Monocytes provide an ideal culture model for the study of immunology. These human CD14+ Monocytes are purified... Read More
-
Human CD19+ B-Cells
Cat.-Nr: HC-0031
Lifeline® Human CD19+ B Cells provide an ideal culture model for the study of immunology. These normal human CD19+ B Cells are purified from... Read More
-
Human CD3+ Pan T-Cells (10M)
Cat.-Nr: HC-0027
Human CD3+ Pan T Cells are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability. Human CD3+ Pan T Cells are... Read More
-
Human CD3+ Pan T-Cells (5M)
Cat.-Nr: HC-0026
Human CD3+ Pan T Cells provide an ideal culture model for the study of immunology, immunotherapy, autoimmunity, cancer, cell-based therapies, and... Read More
-
Human CD3+ T-Cells - Complete Kit (10M)
Cat.-Nr: LL-0077
The CD3+ Pan T Cell 10M System Complete Kit includes: Human CD3+ Pan T Cells with 10 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
-
Human CD3+ T-Cells - Complete Kit (5M)
Cat.-Nr: LL-0076
The CD3+ Pan T Cell 5M System Complete Kit includes: Human CD3+ Pan T Cells with 5 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with... Read More
-
Human CD4+ T-Cells
Cat.-Nr: HC-0028
Lifeline® Human CD4+ T Cells provide an ideal culture model for the study of immunology. These human CD4+ T Cells are purified from leukapharesed... Read More
-
Human CD8+ T-Cells
Cat.-Nr: HC-0029
Lifeline® Human CD8+ T Cells provide an ideal culture model for the study of immunology. Human CD8+ T Cells are purified from leukapharesed... Read More
-
Human Cervical Epithelial Cells
Cat.-Nr: FC-0080
Lifeline® Normal Human Cervical Epithelial Cells (HCxEC), when grown in Lifeline® ReproLife™ CX Medium, provide an ideal serum-free culture model... Read More
-
Human Corneal Epithelial Cells
Cat.-Nr: FC-0029
Lifeline® normal Human Corneal Epithelial Cells (HCEC), when grown in Lifeline® OcuLife™ Medium, provide an ideal serum-free culture model for... Read More
-
Human Coronary Artery Endothelial Cells - CAEC
Cat.-Nr: FC-0032
Lifeline® Normal Human Coronary Artery Endothelial Cells (HCAEC), when grown in Lifeline® VascuLife® Medium, provides an ideal culture model, with... Read More
-
Human Coronary Artery Smooth Muscle Cells - CASMC
Cat.-Nr: FC-0031
Lifeline® normal Human Coronary Artery Smooth Muscle Cells (HCASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
-
Human Dermal Microvascular Endothelial Cells – Adult
Cat.-Nr: FC-0039
Lifeline® normal Human Microvascular Endothelial Cells – Adult, when grown in VascuLife® VEGF-Mv Medium, provide an ideal low-serum culture... Read More
-
Human Endometrial Uterine Epithelial Cells
Cat.-Nr: FC-0078
Lifeline® Normal Human Endometrial (Uterine) Epithelial Cells, when grown in Lifeline® ReproLife™ Medium, provide an ideal serum-free culture... Read More
-
Human Epidermal Keratinocytes - 10-Donor Pool
Cat.-Nr: FC-0064
Lifeline® normal Human Epidermal Keratinocytes (HEK), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for... Read More
-
Human Fallopian Tube Epithelial Cells
Cat.-Nr: FC-0081
Lifeline® Normal Human Fallopian Tube Epithelial Cells , when grown in Lifeline® ReproLife™ Medium, provide an ideal serum-free culture model for... Read More
-
Human Gingival Fibroblasts
Cat.-Nr: FC-0095
Lifeline® normal Human Gingival Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
-
Human Iliac Artery Endothelial Cells
Cat.-Nr: FC-0028
Lifeline® Iliac Artery Endothelial cells, when grown in Lifeline® VascuLife ® culture medium, provide a low-serum culture ideal for the study of... Read More
-
Human Lobar Bronchial Epithelial Cells
Cat.-Nr: FC-0054
Normal Human Lobar Bronchial Epithelial Cells provide an ideal model for the studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology... Read More
-
Human Lung Fibroblast Cells
Cat.-Nr: FC-0049
Lifeline® normal Human Primary Lung Fibroblasts provide an ideal cell system to: Study wound healing, toxicology, cancer, or basic cell... Read More
-
Human Lung Smooth Muscle Cells
Cat.-Nr: FC-0046
Lifeline® normal Human Lung Smooth Muscle Cells, when grown in VascuLife® SMC Medium, provide an ideal culture model for the study of angiogenesis,... Read More
-
Human Mammary Epithelial Cells, Female
Cat.-Nr: FC-0065
Lifeline® normal Human Mammary (Breast) Epithelial Cells (HMEC) – female, when grown in Lifeline® MammaryLife™ Medium, provide an ideal... Read More
-
Human Mammary Epithelial Cells, Male
Cat.-Nr: FC-0063
Lifeline® Normal Human Mammary Epithelial Cells (HMEC) – Male, when grown in Lifeline® MammaryLife™ Medium, provide an ideal serum-free culture... Read More
-
Human Mesenchymal Stem Cells - Adipose
Cat.-Nr: FC-0034
Lifeline® Normal Human Mesenchymal Stem Cells-Adult (HMSC-Ad) provide an ideal culture model for the study of adult stem cell biology and... Read More
-
Human Mesenchymal Stem Cells Wharton´s Jelly
Cat.-Nr: FC-0020
Lifeline® Normal Human Mesenchymal Stem Cells — Wharton’s Jelly (HMSC-WJ) provide an ideal culture model for the study of multipotent stem cell... Read More
-
Human Neonatal Dermal Fibroblasts - HDFneo
Cat.-Nr: FC-0001
Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human... Read More
-
Human Neonatal Dermal Fibroblasts Xeno-Free
Cat.-Nr: FC-0037
Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal... Read More
















































