- Species: Human
- Cell Type: Fibroblasts
- Tissue: Cardiovascular
- Additional Attributes: Primary Cells
- Features:Single donor, cryopreserved as secondary cells
Lifeline® Normal Human Cardiac Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell cultures, or as a model to study wound healing, toxicology, or basic cell biology.
Lifeline® Cardiac Fibroblasts have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest purity, viability, and plating efficiency.
Our fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
Our Cardiac Fibroblasts are not exposed to antimicrobials or phenol red when cultured in Lifeline® FibroLife® medium. Lifeline offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation. A vial of Gentamicin and Amphotericin B (GA; LS-1104) is provided with the purchase of FibroLife® S2 (LL-0011) Medium Complete Kit for your convenience. The use of GA is recommended to inhibit potential fungal or bacterial contamination of eukaryotic cell cultures. Phenol Red (LS-1009) may be purchased, but is not required.
For an overview of available human primary fibroblasts from different tissues and recommended culture media (click).
Lifeline Cell Technology™