Benefits of filtered Collagenase
Save time. Reduce handling. Improve consistency.
Filtered collagenase is passed through a 0.22 µm membrane before being lyophilized in autoclaved vials. This eliminates the need for an additional filtration step after reconstitution, saving preparation time and reducing handling.
- Convenience: Ready for reconstitution without additional laboratory filtration.
- Reduced contamination risk: Fewer open handling steps help minimise opportunities for contamination.
- Consistent preparation: A defined filtration step during manufacturing helps standardise enzyme preparation.
- Potentially better recovery: Avoids potential enzyme losses during additional filtration.
Animal-free options for biopharmaceutical applications
For biopharmaceutical research and bioprocessing, animal-free enzymes can help minimise the risk of introducing animal-derived pathogens. We offer animal-free collagenases (Types A–D), purified animal-free collagenase and STEMxyme® collagenase/neutral protease blends for applications where animal-derived components must be avoided. Selected products are also available in 0.22 µm-filtered formats.
Not sure which enzyme to choose? Consult our Collagenase Wiki for guidance on collagenase types, secondary protease activities and tissue-specific applications.
The table below lists available filtered and non-filtered collagenase preparations to help you find the right format for your workflow.
| Name | Code for filtered Collagenase | Code for non-filtered Collagenase |
| Collagenase, Type 1 | CLSS-1 | CLS-1 |
| Collagenase, Type 2 | CLSS-2 | CLS-2 |
| Collagenase, Type 3 | CLSS-3 | CLS-3 |
| Collagenase, Type 4 | CLSS-4 | CLS-4 |
| Collagenase, Type 5 | CLSS-5 | CLS-5 |
| Collagenase, Type 6 | CLS-6 | |
| Collagenase, Type 7 | CLS-7 | |
| Collagenase, Purified: high Collagenase activity and minimal secondary proteolytic activitie | CLSPANK (a component of the Neonatal Cardiomyocyte Isolation System (NCIS) Kit) | CLSPA |
| Collagenase/Elastase Vial: Mix of Collagenase Type 1 (≥20,000 units) and Elastase (30 units)/vial; component of Hepatocyte Isolation System (HIS) Kit | Collagenase/Elastase (CLSH) | |
| Animal-free Collagenase | ||
| Collagenase Animal-Free, Type A | CLSAFAS | CLSAFA |
| Collagenase Animal-Free, Type B | CLSAFBS | CLSAFB |
| Collagenase Animal-Free, Type C | CLSAFCS | CLSAFC |
| Collagenase Animal-Free, Type D | CLSAFD | |
| STEMxyme®1, Collagenase/Neutral Protease (Dispase®): Mix of Animal Free Collagenase Type B & Animal Free Neutral Protease/Dispase® (NPRO) | STZ1 | |
| STEMxyme®2, Collagenase/Neutral Protease (Dispase®): Mix of Animal Free Collagenase Type B & Animal Free Neutral Protease/Dispase® (NPRO) with higher associatied protease (Caseinase) activity | STZ2 | |
| Collagenase, Purified, Animal Free | CLSAFP |
More filtered enzymes for tissue dissociation:
Other enzymes used in tissue dissociation are also available in filtered formats:
- Elastase: lyophilized powder (Code: ESL) or suspension (Code: ES)
- DNAse I: lyophilized powder (Code: DCLS) or DNAse I (Code: D2) as component of the Papain Dissociation System (PDS Kit)
- Trypsin: lyophilized powder (Code: TRLS)
- Papain: suspension (Code: PAP) or lyophilized powder (Code: PAP2) as component of the Papain Dissociation System (PDS Kit)
