Description
This Elastase is prepared from porcine pancreas. It is extensively used in tissue and cell
dissociation procedures.
Elastase is effective in the isolation of Type II lung cells. Because elastin is
found in the highest concentrations in the elastic fibers of connective tissues, elastase is frequently used
to dissociate tissues which contain extensive intercellular fiber networks. The viscous extracellular ground
substance in which connective tissue cells and fibers are embedded is a complex mixture of various
glycoproteins. Given the complexity of this matrix, proteolytic digestion proceeds best when elastase is
used in concert with other enzymes such as collagenase, trypsin and chymotrypsin.
2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells.
SUPPLIER:
Worthington Biochemical
STATUS:
In Stock
SIZE:
100 mg
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