- Additional Attributes: Proteolytic Enzymes
- Specific Attributes: Animal-free
- Donor / Source:Bacillus polymyxa
- Activity:≥4 units per mg dry weight
Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.
Characteristics of Neutral Protease (Dispase®) from Bacillus polymyxa:
Molecular Weight: 36 kda.
pH Optimum: Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0.
Specificity: Non-specific cleavage of peptide bonds containing leucine and phenylalanine.
Activators: Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+
Inhibitors: EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals
Application(s): Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.