26 Assays Using PureCol Collagen

Collagen is the most abundant protein in the body and is thus the most widely used ECM protein in research across hundreds of various assays.

PureCol® Type I Collagen is the most popular collagen product, being 3 mg/ml of type I atelocollagen (>99.9% pure), and is the product referenced in each of the assays below. Other ABM Collagen products may be substituted in these assays if different mechanical properties of the collagen are desired (ie. use Telocollagen for stiffer hydrogels to evaluate changes in migration related to stiffness). Descriptions of some of the assays are found below the chart.

Assay Title Reference
Boyden Chamber https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Spheroid Invasion https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Single Cell Motility https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Cell Viability https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991007/
Aortic Ring https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907187/
3D Chemotaxis https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836243/
Cell Line Generation https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812522/
Collagen Migration https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751638/
Cell Proliferation https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858314/
Air-Liquid Interface https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651658/
Gel Contraction https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556235/
3D Migration https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523719/
Tumor-On-Chip 3D Motility https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534269/
In Vitro Granuloma Models https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486295/
Injectable Gels https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438067/
3D Cytotoxicity co-culture https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410835/
Invasion https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383389/
CRT (Calreticulin) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436107/
Cell Binding https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195475/
Melanoma Spheroid Invasion https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115293/
Stiffness https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428240/
LH Enzymatic Activity https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055892/
In Vitro 3D Cyst https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251518/
Scratch/Wound Healing https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
Tube Formation https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
Permeability https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873778/

(published 01/26/23: Advanced BioMatrix – 26 Assays Using PureCol Collagen)


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On June 02, 2022, Katharina, daughter of our scientific sales representative Monika Kleu participated in the “Alpe d’HuZes” , a unique sporting event, that raises money for cancer research and for improving the quality of life of people suffering from cancer.
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Chromatographically purified for higher specific activity. Same as Code: ZF except assayed using NAD. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities 0.02 %, 0.003 %, 0.002 % and 0.002 % respectively. A suspension in 3.7 M ammonium sulfate.

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