Subculture & Reagents


Subculture & Reagents for optimal Cell Culture

Our Subculture & Reagents product line plays a vital role in optimizing growth conditions and providing essential propagation reagents for cell culture experiments.

Furthermore, they facilitate the seamless transition of cells from one vessel to another, ensuring their viability and functionality throughout the process.

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Specific Basal Media for a diverse array of Cell Types

A broad spectrum of Basal Media is available to support the growth and maintenance of various cell types.
From male and female reproductive cells to dermal cells such as keratinocytes and endothelial cells, specialized media formulations provide the optimal nutrient and growth factor environment for specific cell populations. These tailored media formulations promote cell health, proliferation, and functionality, ensuring reliable and reproducible results in cell culture experiments.

Single LifeFactors and LifeFactor Kits: Tailored Supplements for Basal Media

To further customize cell culture conditions, LifeFactors and LifeFactor Kits offer a convenient way to supplement the various Basal Media with specific additives and supplements. These kits contain carefully curated components designed to enhance cell growth, specific cellular functions, and partly also differentiation. Whether optimizing culture conditions for a particular cell type or investigating specific biological processes, LifeFactors and LifeFactor Kits provide researchers with the flexibility to tailor their cell culture protocols to their unique experimental needs.

Take advantage of our Growth Media Complete Kits, containing the optimized Basic Media and  optimized LifeFactor Kit.

Trypsin-EDTA for Effective Cell Harvesting

Among the most essential subculture reagents is Trypsin-EDTA, a potent enzyme mixture used to detach adherent cells from the culture surface. By cleaving cell adhesion proteins,Ttrypsin-EDTA facilitates the gentle detachment of cells, ensuring high cell yields with minimal cellular stress. This reagent is indispensable for routine passaging of adherent cell lines and the establishment of new cultures.

Xeno-Free Trypsin-EDTA and more…

For researchers seeking to minimize the risk of introducing animal-derived components into their cell cultures, xeno-free trypsin-EDTA offers a viable solution. Xeno-free trypsin-EDTA provides the same robust performance as traditional formulations while meeting the stringent requirements of animal component-free cell culture systems. Xeno-Free Trypsin-EDTA contains human serum.

Also have a look at our Xeno-Free Human Primary Cells and Cell Culture Media.


  • PBS w/o Ca / Mg

    Cat.-Nr: CM-0001

    Phosphate-buffered saline (PBS) maintains a constant pH, and its ion concentration and osmolarity matches that of human cells. PBS is used to remove... Read More

  • TrypKit Xeno-Free

    Cat.-Nr: LL-0043

    TrypKit ™ Xeno-Free contains: CM-0001, PBS XF, 500 mL CM-0046 Trypsin EDTA XF, 100 mL CM-0047 Trypsin Neutralizing Solution XF, 100 mL

  • TrypKit-100 ml

    TrypKit-100 ml

    Cat.-Nr: LL-0013

    Large TrypKit includes: Phosphate Buffered Saline (PBS) — 500 mL 0.05% Trypsin/0.02% EDTA (phenol red-free) — 100 mL Trypsin Neutralizing... Read More

  • Trypsin 0.05% EDTA 0.02% (phenol red free)

    Cat.-Nr: CM-0017

    Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More

  • Trypsin Neutralizing Solution

    Cat.-Nr: CM-0018

    Trypsin is an enzyme that breaks down proteins to dissociate cells from the vessel in which they’re being cultured.

  • Trypsin Neutralizing Solution Xeno-Free

    Cat.-Nr: CM-0047

    Trypsin neutralizing solution xeno-free acts as an enzyme to help dissociate cells from their culture vessel, and contains no non-human... Read More

  • Trypsin/EDTA Xeno-Free

    Cat.-Nr: CM-0046

    Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More