Overview
- Species: Other
- Specific Attributes: Collagen
- Additional Attributes: Collagen Hybridizing Peptide
Biotin Conjugate
Cat.-Nr.: 5265-60UG
Description
Collagen Hybridizing Peptides (CHP) conjugates from Advanced BioMatrix are labeled with sulfo-Cyanine3 for fluorescence detection.
The triple helix is the hallmark protein structure of collagen. During tissue remodeling, the triple helical collagen molecules are degraded by specific proteases (e.g., MMP or cathepsin K) and become unfolded at body temperature. The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to such denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). By sharing the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen strands, in a fashion that is similar to a DNA fragment annealing to its complimentary DNA strand during PCR. CHP is an extremely specific probe for unfolded collagen molecules: it has negligible affinity to intact collagen molecules due to the lack of binding sites; it is also inert towards non-specific binding because of its neutral and hydrophilic nature.
SUPPLIER:
Advanced BioMatrix
STATUS:
In Stock
SIZE:
60 UG
References
- References for Collagen Hybridizing Peptides:
- Targeting and mimicking collagens via triple helical peptide assemblies. Current Opinion in Chemical Biology, 2013, 17, 968–975.
- In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano, 2017, 11, 9825–9835.
- Targeting collagen strands by photo-triggered triple-helix hybridization. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109, 14767–14772.
- Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials, 2018, 183, 67–76.
- Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjugate Chemistry, 2013, 24, 9–16.
- Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nature Communications, 2017, 8, 14913, doi: 10.1038/ncomms14913.
- Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide. Acta Biomaterialia, 2017, 53, 268–278.
LAP – Visible Light Photoinitiator