- Species: Bovine
- Additional Attributes: 3D Hydrogels, Coating, Collagen, Extracellular Matrix
- Specific Attributes: Atelocollagen/pepsin treatment, steril, Type I
- Features:3 mg/ml
PureCol® collagen (bovine collagen, atelocollagen) is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I atelocollagen with the remainder being comprised of Type III collagen. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.
For extensive users of this product: The PureCol® collagen is available in a 1.000 ml unit, too. (please contact us)
Summary of available bovine collagen type I products:
For an overview of Type I Collagen (Atelocollagen & Telocollagen) products (click)
How is the purity of the PureCol® determined ?
The purity of PureCol® collagen is determined by SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis in conjunction with bacterial collagenase sensitivity and silver staining techniques with a method sensitivity of 99.9%. It was found that PureCol® collagen is 95 to 98% Type I collagen and the remainder being comprised of Type III collagen.
SDS polyacrylamide gel electrophoresis demonstrates the presence of alpha, beta and gamma components in an appropriate ratio of approximately 40:30:30, respectively. PureCol® collagen is a native collagen as judged by polarimetry and trypsin sensitivity although the product does contain a low percentage of collagen fragments or shortened helices.
Conclusion: With the test method sensitivity of 99.9% (SDS-PAGE gel electrophoresis in conjunction with bacterial collagenase sensitivity and silver staining techniques) and no other proteins present in the preparation, it can be concluded that the purity of the PureCol® collagen is 99.9%.
What is the differnce between Vitrogen and PureCol® ?
There is no difference. Vitrogen was the old tradename, and PureCol® is the new tradename.
What is the viscosity of PureCol® ?
The viscosity of PureCol® is ~32 cp.
How you determine the collagen concentration ?
We primarily use the Biuret method, but we also use BCA, AAA, and hydroxyl-proline assays.
Is my collagen okay, if it was frozen, or left at room temperature ?
– Collagen solutions that are frozen tend to have issues forming 3D hydrogels, and will likely not work. The solutions should still be good for 2D coatings.
– Collagen solutions that are left out at room temperature for extended periods of time may show signs of degradation, which will affect the formation of 3D hydrogels. It is likely still fine for 2D coatings.
Our recommendation is this: If you are using the product directly for a publication, we highly suggest buying a new bottle if the one you have was compromised.