DNase & Related Products
In molecular research, DNases play vital roles in DNA degradation and cleavage, while DNA and Histones are fundamental components in understanding genetic mechanisms and chromatin organization.
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Deoxyribonuclease I (DNase I) is an enzyme that catalyzes the hydrolysis of phosphodiester bonds in DNA molecules. This results in the cleavage of DNA into smaller fragments. It is commonly used in molecular biology and biochemistry research for various applications, such as:
- removal of contaminating genomic DNA from RNA preparations, e.g. prior to RT-PCR.
- DNA fragmentation for DNA library construction.
- preparation of single-cell suspensions by degradation of extracellular DNA released from lysed cells.
DNase I is mostly purified from bovine pancreas. These enzymes are available in different compositions, e.g. free of ribonuclease and protease (Code: DPRFS and DPRF), filtered (Code: DCLS and D2) and partially (Code: DSV, DP and DPB) or chromatographically (Code: DPFF and D) purified.
Animal Free DNase I
In addition, for sensitive applications and for industries where stringent quality and regulatory requirements must be met, purified recombinant DNase I products are available. These are produced in genetically engineered yeast (Pichia pastoris). Therefore, these DNase I (Code: DR1, DR1S) are free of RNases, proteases and animal components (Animal Free, AF). The bioprocess grade DNase I (Code: DR2) is Animal Free.
In contrast, DNase II is purified from porcine spleen (Code: HDA, HDAC and HDACS). It has applications in studies focusing on apoptotic cell death and lysosomal DNA degradation research.
DNAs from a variety of sources
DNA-cellulose is a type of chromatography resin used in biochemistry and molecular biology for the purification and isolation of DNA-binding proteins. It consists of cellulose beads to which DNA molecules are covalently attached. DNA-cellulose chromatography takes advantage of the specific interactions between DNA and proteins, allowing researchers to separate proteins based on their affinity for DNA. This technique is commonly used to purify transcription factors, enzymes involved in DNA replication and repair, and other DNA-binding proteins for further study. We offer double-stranded (Code: DNACELDS) and sinlge-stranded DNA-cellulose (Code: DNACELSS) from calf thymus.
Furthermore, we offer DNA from
- Calf Thymus: unaltered (Code: DNA),
- Salmon Testes: unaltered (Code: SDNA) or fragmented (Code: SDNAD)
- Phage Lambda: unaltered (Code: DNAL) and endonuclease digested with Hind III (Code: DNALHIND), EcoRI (Code: DNALECOR), and BstE II (Code: NDALBSTE)
- E. coli (Code: DNAEC) and
- Clostridium perfringens (Code: DNACP).
Histones and Nucleohistones
Histones, proteins located in eukaryotic cell nuclei, play a crucial role in packaging and organizing DNA into nucleosomes. Our product range includes Histones from calf thymus, available in dried (Code: H) and lyophilized (Code: HLY) forms. Additionally, we offer Nucleohistones from calf thymus (Code: NHL), where histone proteins are complexed with DNA.
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D - Deoxyribonuclease I
Cat.-Nr: LS002004
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.
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D - Deoxyribonuclease I
Cat.-Nr: LS002006
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.
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D - Deoxyribonuclease I
Cat.-Nr: LS002007
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.
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DCLS - Deoxyribonuclease I, filtered
Cat.-Nr: LS002058
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
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DCLS - Deoxyribonuclease I, filtered
Cat.-Nr: LS002060
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
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Deoxyribonuclease I, Recombinant, Animal Free, Bioprocess Grade
Cat.-Nr: LS006320
Chromatographically purified AF bioprocessing grade. Supplied as a lyophilized powder containing glycine as a stabilizer. For the removal of DNA in... Read More
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Deoxyribonuclease I, Recombinant, Animal Free, Bioprocess Grade
Cat.-Nr: LS006322
Chromatographically purified AF bioprocessing grade. Supplied as a lyophilized powder containing glycine as a stabilizer. For the removal of DNA in... Read More
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Deoxyribonuclease I, Recombinant, Animal Free, Bioprocess Grade
Cat.-Nr: LS006323
Chromatographically purified AF bioprocessing grade. Supplied as a lyophilized powder containing glycine as a stabilizer. For the removal of DNA in... Read More
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DPFF - Deoxyribonuclease I
Cat.-Nr: LS006328
Chromatographically purified. A lyophilized powder containing glycine as a stabilizer. Contains ≤ 0.0005% RNase.
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DPFF - Deoxyribonuclease I
Cat.-Nr: LS006330
Chromatographically purified. A lyophilized powder containing glycine as a stabilizer. Contains ≤ 0.0005% RNase.
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DPRF - DNase I, RNase & Protease free
Cat.-Nr: LS006331
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vials. Each 10,000 unit vial contains 2 mg... Read More
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DPRF - DNase I, RNase & Protease free
Cat.-Nr: LS006333
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vials. Each 10,000 unit vial contains 2 mg... Read More
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DPRF - DNase I, RNase & Protease free
Cat.-Nr: LS006338
Purified from bovine pancreas.Molec.Bio.Grade. Chromatograph. purified to remove RNase and protease. Lyoph.in vials. =2,000 Ku units per mg dry weight
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DPRFS - Deoxyribonuclease I, Rnase & Protease free
Cat.-Nr: LS006344
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per... Read More
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DPRFS - DNase I, Rnase & Protease free
Cat.-Nr: LS006342
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per... Read More
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DR1 - Deoxyribonuclease I, Recombinant
Cat.-Nr: LS006362
Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and proteases. Chromatographically purified. A... Read More
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DR1 - DNase I, Recombinant
Cat.-Nr: LS006361
Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and proteases. Chromatographically purified. A... Read More
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DR1S - DNase I, Recombinant, Solution
Cat.-Nr: LS006353
Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases & proteases. Chromatographically purified. A liquid... Read More
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DR1S - DNase I, Recombinant, Solution
Cat.-Nr: LS006355
Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases & proteases. Chromatographically purified. A liquid... Read More
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DSV - Deoxyribonuclease I, Standard Vial
Cat.-Nr: LS002172
Lyophilized in vials for assay standardization. Labeled to show established activity. Not suitable for assays at neutral pH.
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DSV - Deoxyribonuclease I, Standard Vial
Cat.-Nr: LS002173
Lyophilized in vials for assay standardization. Labeled to show established activity. Not suitable for assays at neutral pH.
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HDAC - Deoxyribonuclease II
Cat.-Nr: LS005410
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
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HDACS - Deoxyribonuclease II
Cat.-Nr: LS005416
Chromatographically prepared. A solution in 50% glycerol.
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HDACS - Deoxyribonuclease II
Cat.-Nr: LS005418
Chromatographically prepared. A solution in 50% glycerol.