Primary Cells & Culture Media
Isolated Primary Cells and selective Cell Culture Media in Experimental Research
Utilizing isolated primary cells in conjunction with carefully optimized cell culture media aims to replicate in vivo conditions, thereby enhancing the physiological relevance of studying cellular behaviors and responses.
Therefore, primary cell culture is the first choice when interested in:
- Disease Modelling and Mechanistic Studies: Isolated primary cells serve as essential models for studying disease mechanisms and pathophysiology. Cultures of healthy and diseased lung epithelial cells (e.g. bronchial/tracheal, small airway), or immune cells, among others, enable researchers to elucidate the underlying molecular pathways driving diseases such as pathogenesis and dysfunction of epithelial tissues and autoimmune conditions.
- Drug Discovery and Development Studies: Primary cell cultures are invaluable tools in drug discovery, allowing researchers to assess the efficacy, toxicity, and pharmacological properties of candidate compounds in a biologically relevant context.
Primary cell culture systems provide researchers with more physiologically relevant models for studying complex biological processes, disease mechanisms, and drug responses.
3D Culture Models: From Organoids, Co-Culture Systems and Organ-on-a-Chip Technology
Organoid and spheroid cultures as well as co-culture systems represent advanced three-dimensional models that mimic the complexity of tissues and organs in vitro.These approaches result in more complex and physiologically relevant systems compared to traditional monolayer cultures, allowing researchers to study cellular interactions, tissue development, disease mechanisms, and drug responses within a setting that closely mimics in vivo conditions.
Three-dimensional, multilineage human skin models—an area where CellSystems® has historically acquired extensive experience—serve as a basis for the reproduction and study of various skin diseases, as well as their treatment in vitro. Furthermore, these models have significantly advanced research in critical areas such as wound healing, and burn therapy. Additionally, the emergence of Organ-on-a-Chip Technology has provided captivating insights into the complex microenvironment and physiological responses of human organs in vitro. In a recent publication, primary cells, culture media, and extracellular matrix proteins were utilized in a microfluidic two-channel co-culture to shed light on the dynamics of the vaginal microbiome for understanding female reproductive tract health and disease.
For a comprehensive overview about our extracellular matrices, adhesion molecules and bioprinting materials, visit Matrix Proteins & Bioprinting.
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DermaLife K Complete Medium - Fully Supplemented
Cat.-Nr: LM-0027
DermaLife K Complete Medium is a fully supplemented frozen medium optimized for the culture of human keratinocytes; offering ease of use and an... Read More
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DermaLife K, Keratinocyte Growth Medium Complete Kit
Cat.-Nr: LL-0007
DermaLife K, Keratinocyte Growth Medium Complete Kit is optimized for culturing Human Epidermal Keratinocytes in a serum-free environment. DemaLife K... Read More
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Human Oral Keratinocytes - Gingiva
Cat.-Nr: FC-0094
Lifeline® normal Human Oral Keratinocytes (Gingiva), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for the... Read More
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AdipoLife Basal Medium
Cat.-Nr: LM-0021
AdipoLife™ is a new medium based on the formulas found in academic literature for the differentiation of Human Mesenchymal Stem Cells into... Read More
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AdipoLife Complete Kit
Cat.-Nr: LL-0050
AdipoLife™ DfKt™-1 Adipogenesis Medium is optimized for the differentiation of Human Adipose Mesenchymal Stem Cells (HMSC-Ad) and Pre-Adipocytes... Read More
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AdipoLife WJ Complete Kit
Cat.-Nr: LL-0059
AdipoLife™ DfKt™-2 Adipogenesis Medium differentiates Human Mesenchymal Stem Cells (HMSC) derived from bone marrow (BM) and Wharton’s Jelly... Read More
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BronchiaLife B/T Epithelial Airway Medium Complete Kit
Cat.-Nr: LL-0023
BronchiaLife™ B/T Epithelial Airway Medium Complete Kit is optimized for the serum-free culture of Human Airway Epithelial Cells, including... Read More
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BronchiaLife LifeFactors Kit
Cat.-Nr: LS-1047
This kit contains the associated supplements and growth factors for use with Human Airway Epithelial Cells and BronchiaLife™ Basal Medium. With... Read More
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BronchialLife Basal Medium
Cat.-Nr: LM-0007
BronchiaLife™ Basal Medium is optimized for the serum-free culture of Human Airway Epithelial Cells (including Bronchial/Tracheal Epithelial, Lobar... Read More
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CD14+ Monocytes System Complete Kit
Cat.-Nr: LL-0080
CD14+ Monocytes with RPMI Medium in a Complete Kit. The CD14+ Monocytes System Complete Kit includes: 5M CD14+ cryopreserved Monocytes RPMI 1640-FBS... Read More
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CD19+ B Cells System Complete Kit
Cat.-Nr: LL-0081
Lifeline® CD19+ B Cells System Complete Kit includes: 5 million CD19+ B Cells and RPMI 1640-FBS Cell Culture Medium Complete Kit with Frozen Basal... Read More
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CD4+ T Cells System Complete Kit
Cat.-Nr: LL-0078
Lifeline® Human CD4+ T Cells System Complete Kit includes: Human CD4+ Helper T Cells cryopreserved at 5.000.000 cells per vial (HC-0028) and RPMI... Read More
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CD8+ T Cells System Complete Kit
Cat.-Nr: LL-0079
Lifeline® CD8+ T Cells System Complete Kit includes: 5M CD8+ Cytotoxic T Cells (HC-0029) and RPMI1640-FBS Medium Complete Kit with frozen basal... Read More
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ChondroLife Complete Chondrogenesis Medium
Cat.-Nr: LM-0022
ChondroLife™ Complete Chondrogenesis Frozen Medium is a new medium optimized for the differentiation of Human Mesenchymal Stem Cells into... Read More
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DermaLife Basal Medium
Cat.-Nr: LM-0004
DermaLife Basal Medium is optimized for the culture of Human Dermal Epithelial Cells (including Keratinocytes, Adult Melanocytes, and Neonatal... Read More
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DermaLife Calcium-Free Basal Medium
Cat.-Nr: LM-0008
DermaLife Calcium-Free Basal Medium is a serum-free and calcium-free medium optimized specifically for the culture of Human Primary... Read More
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DermaLife K Ca Free Complete Kit
Cat.-Nr: LL-0029
DermaLife K Calcium Free Medium Complete Kit is a serum-free and calcium-free medium. Calcium Chloride has been omitted from the Basal Medium... Read More
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DermaLife K LifeFactors Kit
Cat.-Nr: LS-1030
Supplements and growth factors for use with Human Keratinocytes and DermaLife Basal Medium or DermaLife Calcium-Free Basal Medium. LifeFactors® are... Read More
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Dermalife M LifeFactors Kit
Cat.-Nr: LS-1041
Supplements and growth factors for use with Human Melanocytes -Neonatal and DermaLife Basal Medium. The LifeFactors® are packaged in a convenient... Read More
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DermaLife M, Melanocyte Growth Medium Complete Kit
Cat.-Nr: LL-0027
DermaLife® M, Melanocyte Culture Medium Complete Kit is a low serum, PMA-free, Cholera toxin-free medium optimized for the culture of Human... Read More
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DermaLife Ma LifeFactors Kit
Cat.-Nr: LS-1063
This kit includes the associated supplements and growth factors called “LifeFactors®” for use with Human Adult Melanocytes and DermaLife Basal... Read More
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DermaLife Ma, Melanocyte Growth Medium Complete Kit (adult)
Cat.-Nr: LL-0039
DermaLife® Ma, Melanocyte Growth Medium Complete Kit (adult) has been optimized for culturing Adult Human Epidermal Melanocytes in a low-serum, PMA... Read More
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Diseased Human Small Airway Epithelial Cells
Cat.-Nr: FC-0067
Diseased Human Small Airway Epithelial Primary Cells — Asthma + COPD (Chronic Obstructive Pulmonary Disease).These primary cells have been... Read More
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Extract P™ Bovine Pituitary Extract, New Zealand Origin
Cat.-Nr: CM-0097
Extract P™ Bovine Pituitary Extract (BPE) Supplement Extract P™ is a protein-rich aqueous solution that has been extracted from bovine pituitary... Read More
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FibroLife Basal Medium
Cat.-Nr: LM-0001
FibroLife® basal media has been optimized for the culture of Human Fibroblasts and Mesenchymal Stem Cells-Pre-Adipocytes. FibroLife® provides a... Read More
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FibroLife Fibroblast Serum-Free Medium Complete Kit
Cat.-Nr: LL-0001
FibroLife® Fibroblast Serum-Free Medium Complete Kit is a defined medium optimized for the serum-free culture of fibroblasts. FibroLife® Serum-Free... Read More
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FibroLife S2 Fibroblast Growth Medium Complete Kit
Cat.-Nr: LL-0011
Optimized for Culturing Human Fibroblasts and Mesenchymal Stem Cells-Pre-Adipocytes in a Low-Serum (2% FBS) Environment: FibroLife® S2 supports the... Read More
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FibroLife S2 LifeFactors Kit
Cat.-Nr: LS-1038
Associated supplements and growth factors for use with FibroLife® Basal Medium for low-serum (2%) culture of Human Fibroblasts and Mesenchymal Stem... Read More
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FibroLife Serum-Free Fibroblast LifeFactors Kit
Cat.-Nr: LS-1010
Associated supplements and growth factors for use with Human Fibroblasts and FibroLife® Basal Medium. FibroLife Serum-Free LifeFactors... Read More
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FibroLife Xeno-Free Fibroblast Complete Frozen Medium
Cat.-Nr: LM-0013
FibroLife® Xeno-Free Fibroblast Complete Frozen Medium is a variation of our FibroLife® Cell Culture Medium optimized for culture of fibroblasts... Read More
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FrostaLife Cryopreservation Solution
Cat.-Nr: LM-0015
FrostaLife™ is a solution optimized for the cryopreservation of mammalian cells by Lifeline® cell culture experts. Simply count your cells,... Read More
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HBTEC Air-Liquid Interface Differentiation Medium
Cat.-Nr: LM-0050
HBTEC Air-Liquid Interface Differentiation Medium is a new frozen medium optimized for air-liquid interface mucociliary differentiation of Human... Read More
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Highly Pigmented Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0090
Lifeline® normal Human Epidermal Melanocytes Adult, secondary, highly pigmented (HEMa-HP), when grown in Lifeline® DermaLife Ma Medium, provide an... Read More
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Highly Pigmented Human Neonatal Epidermal Melanocytes
Cat.-Nr: FC-0089
Lifeline® normal Human Epidermal Melanocytes – Neonatal, Secondary, Highly Pigmented (HEMn-HP), when grown in Lifeline® DermaLife M medium,... Read More
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Human Adult Dermal Fibroblasts - HDFad
Cat.-Nr: FC-0024
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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Human Adult Epidermal Keratinocytes - NHEKad
Cat.-Nr: FC-0025
Lifeline® Normal Human Epidermal Keratinocytes adult (HEKa), when grown in Lifeline® DermaLife K Medium, provides an ideal serum-free culture... Read More
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Human Adult Epidermal Melanocytes
Cat.-Nr: FC-0030
Lifeline® Normal Human Epidermal Melanocytes – Adult (HEMa), when grown in Lifeline® DermaLife Ma Medium, provide an ideal low serum culture... Read More
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Human Aortic Endothelial Cells
Cat.-Nr: FC-0014
Lifeline® normal Human Aortic Endothelial Cells, when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture model — with or... Read More
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Human Aortic Smooth Muscle Cells
Cat.-Nr: FC-0015
Lifeline® smooth muscle cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure... Read More
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Human Bladder Apex Epithelial Cells
Cat.-Nr: FC-0040
Lifeline® Normal Human Bladder Apex Epithelial Cells, when grown in UroLife™ Bladder Epithelial Medium Complete Kit, provide an ideal culture... Read More
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Human Bladder Dome Epithelial Cells
Cat.-Nr: FC-0079
Lifeline® Primary Normal Human Bladder Dome Epithelial Cells, when grown in UroLifeTM Culture Medium Complete Kit, provide an ideal culture model... Read More
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Human Bladder Fibroblasts
Cat.-Nr: FC-0050
Lifeline® normal Human Bladder Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
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Human Bladder Smooth Muscle Cells
Cat.-Nr: FC-0043
Lifeline® Normal Human Bladder Smooth Muscle Cells (HBSMC), when grown in Lifeline® VascuLife® SMC Medium, provide an ideal low serum (5%) culture... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 0.5M
Cat.-Nr: ASEBMCD34-C0.5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 1M
Cat.-Nr: ASEBMCD34-C1M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow CD34+ Stem Cells - Single Donor 5M
Cat.-Nr: ASEBMCD34-C5M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mesenchymal Stem Cells - MSC-BM
Cat.-Nr: FC-0057
Lifeline® Human Bone Marrow Mesenchymal Stem Cells (HMSC-BM) provide an ideal culture model for the study of multipotent stem cell biology and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 10M
Cat.-Nr: ASEBMMN-C10M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 20M
Cat.-Nr: ASEBMMN-C20M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More
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Human Bone Marrow Mononuclear Cells (BMMCs) 50M
Cat.-Nr: ASEBMMN-C50M
If you’d like to receive more information about this product, please send us a short message using the “Request Information”-Button below, and... Read More