- Species: Human, Mouse
- Cell Type: Epithelial, Keratinocytes
- Tissue: Skin
- Additional Attributes: Media
- Packaging:Basal Medium plus LifeFactor Kit
- Features:Basal Medium without Calcium
DermaLife K Calcium Free Medium Complete Kit is a serum-free and calcium-free medium.
It’s a customer-specific production under specific conditions (for details, please contact us)
Calcium Chloride has been omitted from the Basal Medium and is supplied as an individual LifeFactor.
DermaLife K has been optimized for the culture of Human Keratinocytes, and supports their growth over at least 15 population doublings. DermaLife Calcium-Free Basal Medium does not include phenol red or antimicrobials.
DermaLife Calcium-Free Basal Medium contains no calcium and will not support cell attachment and growth. Lifeline® calcium chloride supplement gives you the ability to vary the Ca+ concentration to meet your experimental needs. Recommended range of final calcium concentration is 0.02 mM to 0.15 mM, dependent upon cell type.
Add the LifeFactors® to promote guaranteed proliferation and function of cells.
Kit format for formulation flexibility: Offered in a kit format composed of calcium-free basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
Protective packaging: DermaLife Calcium-Free Basal Medium is provided in a light-protected 500 mL bottle. The LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™