- Species: Human, Mouse
- Cell Type: Epithelial Cells, Keratinocytes
- Tissue: Skin
- Additional Attributes: Media
- Packaging:Basal Medium plus LifeFactor Kit
- Features:Basal Medium without Calcium
DermaLife K Calcium Free Medium Complete Kit is a serum-free and calcium-free medium.
It’s a customer-specific production under specific conditions (for details, please contact us)
Calcium Chloride has been omitted from the Basal Medium and is supplied as an individual LifeFactor.
DermaLife K has been optimized for the culture of Human Keratinocytes, and supports their growth over at least 15 population doublings. DermaLife Calcium-Free Basal Medium does not include phenol red or antimicrobials.
DermaLife Calcium-Free Basal Medium contains no calcium and will not support cell attachment and growth. Lifeline® calcium chloride supplement gives you the ability to vary the Ca+ concentration to meet your experimental needs. Recommended range of final calcium concentration is 0.02 mM to 0.15 mM, dependent upon cell type. For example, add 214 µL of the Calcium Chloride LifeFactor (included in this Complete Kit) and achieve 0,06 mM/60 µM CaCl2 final concentration in the supplemented medium.
Add the LifeFactors® to promote guaranteed proliferation and function of cells.
Kit format for formulation flexibility: Offered in a kit format composed of calcium-free basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
Protective packaging: DermaLife Calcium-Free Basal Medium is provided in a light-protected 500 mL bottle. The LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).
Lifeline Cell Technology™