NEW! Bio-Spun™ Scaffolds for 3D Cell Culture Applications

With Bio-Spun™ Scaffolds from BioSurfaces, CellSystems now offers an innovative solution for optimising and standardising complex 3D cell cultures. Produced using a patented electrospinning technology, these scaffolds form a random, nanofibrous 3D structure that closely resembles the extracellular matrix (ECM) of natural tissue.

Comparison of an microscopic picuture of Bio-Spun scaffold material with body's natural scaffold.

Fully animal-free and available in biodegradable or non-biodegradable polymers, the scaffolds promote more physiological cell interactions, help reduce stress responses, and improve the transferability of results to in vivo systems. They also prevent unwanted ECM breakdown and remodelling – a clear advantage over many animal-derived matrices.

Typical applications include:
• Skin models (e.g. irritation, sensitisation, wound healing)
• Airway models (e.g. infection research, inhalation toxicology, drug delivery)
• Models for eye, tumour, skeletal muscle, neurons, blood-brain barrier, liver and more

Key product benefits:
• 100% animal-free
• Choice of biodegradable or non-biodegradable polymers
• Low batch-to-batch variation for reliable, reproducible results

• Saves time and cost in development and testing

Available formats:

Inserts compatible with 6-, 12- and 24-well Transwell Plates, as well as ready-to-use 24- and 96-well High-Throughput Screening Plates

• Materials:
– Non-degradable polymers (PET, PU)
– Biodegradable polymers (PDLGA, PDLGA/PLLA Bilayer)
• Membrane thicknesses:
– PET: 150 μm
– PU: 20 μm
– PDLGA: 10 μm
– PDLGA/PLLA Bilayer: 100 μm

Not sure which scaffold to choose? The Bio-Spun™ Scaffold Overview helps you match the right product to your specific application and tissue model.

NUCxyme™ — High-Performance DNA/RNA Nuclease for Nucleic Acid Removal in Research & Bioprocessing

NUCxyme™ is a highly specific, sequence-independent endonuclease that degrades all forms of DNA and RNA — single-stranded, double-stranded, linear, and circular. It hydrolyzes nucleic acids into 3–5 base oligonucleotides with 5’ monophosphate termini, effectively reducing viscosity from cellular extracts and eliminating residual DNA/RNA in complex samples.

Recombinantly produced in Komagataella phaffii, NUCxyme™ is free of animal-derived materials, endotoxins, and proteases — making it highly suitable for sensitive applications in biotechnology and downstream processing.

Key Features

  • Exceptional Nuclease Activity
    Rapid and complete digestion of linear and circular forms of ssDNA, dsDNA, and RNA
  • High Purity and Safety
    Free from proteases, endotoxins, and animal-derived materials.
  • Yeast-Expressed, Recombinant
    Produced in Komagataella phaffii to avoid contaminants found in bacterial or animal-based sources.
  • Ready-to-Use Liquid Format
    Supplied in 20 mM Tris-HCl, 2 mM MgCl₂, 20 mM NaCl, pH 8.0 with 50% glycerol.

NUCxyme™ is offered in 25 ku and 100 ku formats. Please contact us for bulk or custom quantities.

CytoSoft® – New T-25 and T-75 Flasks Substrates of Varying Rigidity

CytoSoft® T-25 and T-75 flasks provide an increased surface area for passaging cells on an in vivo-like substrate.

 

The CytoSoft® portfolio includes a broad range of formats designed for different phases of research, including:

6-well plates – Initial discovery

24 and 96-well plates – High resolution imaging / publication quality data

T-25 and T-75 flasks – Cell passaging / expansion

 

Advanced BioMatrix (ABM) has acquired the HyStem® line of products from Lineage Cell Therapeutics (LCT)

The TARGATT™-HEK293 Master Cell Line and Knock-in Kit was designed for fast and site-specific knock-in in HEK293 cells, using an easy-to-use gene knock-in approach. The master cell line provided in this kit contains an “attP” integrase-recognition landing pad engineered into the hH11 safe harbor locus in the genome. The kit also contains a cloning plasmid containing a corresponding “attB” sequence into which any gene of interest can be cloned (under control of the CMV promoter or a promoter-of-choice). The expression of the integrase (provided as an integrase plasmid) mediates the stable integration of the transgene into the master cell line (Figure 1). The TARGATT™ integrase technology enables highly efficient (>40 % without enrichment and ~90 % with selection), and site-specific DNA integration without disruption of internal genes. The TARGATT™-HEK293 master cell line can therefore be used for building mammalian cell libraries with site-specific, single transgene gene knock-in and uniform, stable gene expression.

Advantages of using TARGATT™ Master Cell Lines for gene knock-in:

– High efficiency Integration

– Site-specific, stable knock-in cell line generation

– Single copy gene integration into safe harbor locus

– Gene expression from an active, intergenic locus

– Low off-target integration

The TARGATT™-HEK293 master cell line and knock-in kit are suitable for research applications involving directed-evolution of proteins (vaccine development, drug screening, cell-based gene therapy), genome-wide screening, and other stable cell line generation applications. If you are interested to get your own MASTER cell line (including stem cells), please contact us to discuss your project.