Matrix Proteins / Bioprinting

Search for Product

Product categories

Search for Product

Reset

Featured Categories

  • No categories

Matrix Proteins / Bioprinting

The behavior of cells cultured in a three-dimensional (3D) environment more accurately mirrors cellular responses observed in vivo. 3D cell culture systems or so-called 3D models (an in-vitro experimental set-up) better represent the complex microenvironment found within the body’s tissues. Extensive research indicates notable morphological and physiological differences between cells cultured in a 3D environment compared to those in a traditional two-dimensional (2D) culture setup.

The pivotal aspect of 3D culture lies in its additional dimensionality, playing a crucial role in eliciting distinct cellular responses. This dimensionality not only influences the spatial organization of cell surface receptors engaged in interactions with neighboring cells but also imposes physical constraints on the cells themselves. These spatial and physical aspects in 3D cultures significantly impact signal transduction processes, bridging external stimuli to internal cellular responses. Therefore, a 3D model (i.e. skin model, lung model, atherosclerosis model) can mimic important steps for cardiovascular research, lung research, and cancer research. (cell adhesion, invasion of cells, transmigration of cells through an endothelial layer, impact on extracellular molecules)

Moreover, biomolecules such as collagen (atelocollagen and telocollagen), elastin, laminin, adhesion peptides, and adhesion molecules play a crucial role in the structure and function of 3D cultures. These proteins contribute to the formation and maintenance of the extracellular matrix, influencing cell adhesion and promoting a more realistic replication of the natural tissue environment. The stiffness of the 3D environment also contributes to the differentiation of cellular responses.

The rigidity of the substrate (stiffness) to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® plates provide a tool to culture cells on substrates with various rigidities covering a broad physiological range 0.2, 0.5, 2, 8, 16, 32, 64 kPa.

In addition, to analyze the impact of different stiffness of the gel itself, methacrylated matrix proteins can be used (PhotoCol®, PhotoGel®, and  PhotoHA®).

During the last years a new technique was established to build gels with a 3D printer (called bioprinting). These models include the same biomolecules such as collagen (atelocollagen and telocollagen), elastin, laminin, adhesion peptides, and adhesion molecules and gives us a new look inside this fantastic world.