- Species: Synthetic
- Additional Attributes: CytoSoft Substrate (Rigidity) Products
- Packaging:10 x T-25 flasks
- Features:Regidity (elastic modules) 2 kPa
This CytoSoft® T-25 Flask product has a defined elastic modulus (see certificate of analysis) in the bottom of a standard T-25 Flask. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each well that is fully compatible with mammalian cell cultures. The silicone gels are activated and ready to bind to a purified ECM, such as PureCol® type I collagen (#5005) prior to cell addition. The plates are packaged sterilized, and provided with 10 flasks per package.
The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.
The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each well is firmly bonded to the bottom of the well. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.
CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.
CytoSoft coating reagents:
Which cells have been used for CytoSoft ?
For example: Primary Rat Astrocytes, Human Pulmonary Fibroblasts, Human Adipose-Derived Stem Cells, Human Foreskin Fibroblasts, Human Foreskin Fibroblasts, Mesenchymal Stem Cells, Myocytes, Cardiac Fibroblasts, Glial Cells, Neural Cells, Primary Human Dermal Fibroblasts, Murine Primary Palatal Fibroblasts, Longitudinal Smooth Muscle Cells, Human Mesenchymal Stem Cells, Neural Progenitor Cells, Astrocytes, Human Lung Fibroblasts, Human Oral Squamous Cell Carcinoma Cells, Human Conjunctival Fibroblasts
How do you measure the stiffness of the CytoSoft plates ?
To summarize, we have a layer of gold particles on the bottom of a specially prepared slide. We have the silicone on top of those gold particles, and then we add more gold particles on top of the silicone. We then centrifuge the sample at various speeds and measure the relative displacement/movement of the top beads (on the silicone), compared to the bottom beads (attached to the glass bottom). We then use a formula to calculate the youngs modulus of the silicone for that particular sample.
Is it okay if I accidentally freeze CytoSoft plates ?
Do not freeze the CytoSoft® products.
When frozen, there is a good chance that the silicone surface gets hydrolyzed and absorbs moisture, which would inactivate the binding sites and make the product not-usable.
If you froze the product and it is still frozen, warm the CytoSoft® up at 60C with the bag vented. That will minimize the chance of them absorbing moisture – but there is still a chance that they will no longer be functional.
Can I harvest (detach) my cells from the CytoSoft plates directly with Trizol ?
Yes – you can add Trizol directly to the plates.
Do you have questions ?
Please contact us