- Species: Human
- Additional Attributes: Extracellular Matrix
- Specific Attributes: recombinant, steril
- Features:0,5 mg/ml
Vitronectin is a 478 amino acid protein (1-19aa = signal domain) that belongs to a member of the pexin family. Vitronectin is an abundant glycoprotein found in serum and the extracellular matrix. It promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin has been speculated to be involved in hemostasis and tumor malignancy.
Recent publications indicated that coated recombinant human vitronectin protein alone benefits iPS cell generation when combined with E8 culture medium. The product has also been shown to be an excellent coating matrix material for 1 1R-tagged recombinant TF intracellular delivery for protein derived iPS protocol with extremely low levels of non-specific interaction. Recombinant human Vitronectin gene (20-398 aa Fragment) was constructed with codon optimization and expressed in non-fusion protein form in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. Further, coating this recombinant protein at 5 – 10 µg/well (6 well plate in either NutriStem or E8 medium can be used for human iPS cell generation in vitro. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.