Primary Cells
Comprehensive Collection of Ethically Sourced Human Primary Cells
We offer an extensive portfolio of human primary cells, including some rare types, isolated from various tissues such as solid tissues, peripheral blood, cord blood, and bone marrow. All primary cells are obtained under the highest ethical and legal standards and are sourced from a wide variety of donors (including young, old, male, female, and individuals from different ethnicities), each with detailed donor specifications.
Primary Cells from solid tissues
Following isolation, primary cells from solid tissues are cultured in selective media, and harvested in early passages (P0, P1). PCR testing ensures they are negative for HIV-1, HIV-2, HBV, and HCV. Furthermore, cells are rigorously tested to ensure they are free from mycoplasma, bacterial, or fungal contamination, with thorough monitoring conducted after two weeks of cell culture.
With the capacity to function similarly to in vivo cells, these cells serve as invaluable model systems for drug discovery, toxicity testing, gene therapy, cancer research, and more.
Primary Cells from hematopoietic tissues
We also provide primary cells from (mobilized) peripheral blood, cord blood, and bone marrow, collected and frozen without prior culture. Furthermore, marker-defined subsets, such as hematopoietic stem cells, progenitor cells, and immune cells are available. These subsets are isolated either by positive selection of the wanted cell population or depletion of the unwanted fraction.
Tailored Services for Primary Cells
For long-term projects, specific lots of primary cells can be reserved. Custom Cell Isolation services are available for difficult-to-obtain tissues.
Explore our selective Growth Media optimized for cell culture of human primary cells as well as our extensive portfolio of Extracellular Matrices for both 2D and 3D culture.
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HDF, Human Dermal Fibroblasts, adult
Cat.-Nr: FC-0024
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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HDF, Human Dermal Fibroblasts, neonatal
Cat.-Nr: FC-0001
Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human... Read More
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HDFn-XF, Human Dermal Fibroblasts neonatal, Xeno-Free
Cat.-Nr: FC-0037
Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal... Read More
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Human Bladder Fibroblasts
Cat.-Nr: FC-0050
Lifeline® normal Human Bladder Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
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Human Gingival Fibroblasts
Cat.-Nr: FC-0095
Lifeline® normal Human Gingival Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell... Read More
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Human Lung Fibroblast Cells
Cat.-Nr: FC-0049
Lifeline® normal Human Primary Lung Fibroblasts provide an ideal cell system to: Study wound healing, toxicology, cancer, or basic cell... Read More
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Human Prostate Fibroblasts
Cat.-Nr: FC-0099
Lifeline® Normal Human Prostate Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell... Read More
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Human Scleral Fibroblasts
Cat.-Nr: FC-0098
Normal Human Sccleral Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell cultures or... Read More
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Human Uterine Fibroblasts
Cat.-Nr: FC-0076
Lifeline® normal Human Uterine Fibroblast Cells, when grown in FibroLife® S2 Medium, provide ideal In Vitro models for Uterine contraction and... Read More
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Human Vas Deferens Fibroblasts
Cat.-Nr: FC-0052
Lifeline® Vas Deferens Fibroblasts, when grown in FibroLife® S2 culture medium, provide an excellent in vitro model for the study of male... Read More
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Normal Human Cardiac Fibroblasts
Cat.-Nr: FC-0060
Lifeline® Normal Human Cardiac Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell... Read More