- Species: Human
- Cell Type: Fibroblast
- Tissue: Reproductive
- Additional Attributes: Primary Cells
- Features:Single donor, cryopreserved as secondary cells
Lifeline® normal Human Uterine Fibroblast Cells, when grown in FibroLife® S2 Medium, provide ideal In Vitro models for Uterine contraction and relaxation studies, Uterine cancer studies, general Uterine biology studies and specific responses to estrogen, progesterone, relaxin, oxytocin and other female reproductive hormones.
Lifeline® Human Uterine Fibroblasts have been plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency. Our uterine fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-FreeTM Medium and demonstrate optimal growth at rates equal to or greater than classical serum-supplemented media.
These media support the growth of our cells over a period of at least 15 population doublings.
Lifeline® uterine fibroblasts need not be exposed to antimicrobials or phenol red when cultured in FibroLife® Medium. It has long been established in the literature that phenol red acts as a weak estrogen in cell culture media and may stimulate estrogen receptors. This can cause masking effects that could negatively affect studies with human female reproductive cells.
500,000 cells per vial
Uterine fibroblasts are tested for:
Proliferation and Morphology: Normal cell appearance for 15 population doublings
Cell Viability: > = 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma Negative for bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
For an overview of available human primary fibroblasts from different tissues and recommended culture media (click).
Lifeline Cell Technology™