Description
Optimized for Culturing Human Fibroblasts and Mesenchymal Stem Cells-Pre-Adipocytes in a Low-Serum (2% FBS) Environment: FibroLife® S2 supports the growth of these cells in a 2% serum environment at rates equal to or greater than comparable media supplemented with 2% to 10% FBS.
FibroLife® Basal Medium contains no antimicrobials and no phenol red. Antimicrobials and phenol red are not required for eukaryotic cell proliferation, but are available from Lifeline® .
FibroLife® S2 provides an ideal cell system to establish low-serum human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.
Kit format for formulation flexibility: Offered in a kit format comprised of FibroLife® Basal Medium in a light-protected 500 mL bottle, and FibroLife® S2 LifeFactors® Kit (for details link)with associated supplements and growth factors packaged in a convenient gas-impermeable pouch. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
ISO-Style Quality Testing for Reproducible Results
FibroLife® S2 medium is tested for:
Sterility: Negative for bacteria and fungal growth
pH: 7.4 +/- 0.2
Cell Performance: Rate of proliferation and morphology
Osmolality: 270 +/- 10 mOsm
Endotoxin: < 0.5 EU/mL
FibroLife® S2 medium grows fibroblasts and pre-adipocytes in low-serum conditions at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially-available media, FibroLife® S2 medium shows superior proliferation at different seeding densities.
For an overview of available human primary fibroblasts from different tissues and recommended culture media (click).
For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).