Description
Worthington Collagenase Type 5 contains higher collagenase and caseinase activities and is particularly suited for the dissociation of dense connective tissues and collagen-rich extracellular matrices. Typical applications include synovial tissue, articular cartilage, meniscus, tumor tissue, and umbilical cord-derived Wharton’s jelly for the isolation of fibroblast-like synoviocytes (FLS), chondrocytes, mesenchymal stromal cells (MSCs), and other stromal cell populations.
For a detailed comparison of all collagenase types, their enzymatic activity profiles, and guidance on selecting the right preparation for your application, see the Collagenase Wiki.
For guidance on tissue dissociation and a curated collection of published references — covering a broad range of tissues, cell types, and species — request your Tissue Dissociation Guide.
Available Preparations and Pack Sizes
Collagenase Type 5 is available unfiltered (CLS-5) (see below) and as a 0.22 µm membrane-filtered preparation (CLSS-5) – the filtered version is a standard precaution in primary cell isolation workflows to reduce the risk of contamination.
Preparations are stable for 12 months from date of receipt when stored as recommended.
For a complete overview of all available collagenase preparations and ready-to-use tissue dissociation kits, see our full collagenase portfolio.
Partially purified, dialyzed, lyophilized powder. =450 units per mg dry weight. Store at 2-8°C.
