Tissue Dissociation

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Tissue Dissociation

Tissue dissociation, primary cell isolation, as well as cell harvesting of cultured cells are routine processes in various research fields, such as cell biology, neuroscience, immunology, cancer, stem cell and regenerative medicine research. These techniques are essential for obtaining the specific cell types of interest from the full range of available tissues, enabling subsequent experiments and analyses.

Therefore, the primary objective of cell isolation procedures is to maximize the yield of functionally viable, dissociated cells with preserved cell surface antigens, while avoiding cell death and aggregation.

The enzymes used must selectively digest the distinct molecular structures present in the specific tissue, facilitating tissue dissociation while preserving the integrity of essential biomolecules, such as proteins and DNA, for further experimental analyses.

Choosing the right enzymes or enzyme mixes, as well as experimental conditions, is key for optimal results, given that the diverse composition of extracellular matrices in different tissues and developmental stages necessitates tailored approaches.

To receive valuable insights on optimizing your protocols, simply request your digital copy of Worthingtons’ Tissue Dissociation Guide from us. This comprehensive resource consolidates information on standard laboratory procedures, provides a logical experimental framework for developing cell isolation protocols, and includes numerous references specific to various tissues.

Find more information about tissue dissociation enzymes, such as Collagenases, Papain, Trypsin, Elastase, Hyaluronidase, as well as DNases and ready-to-use Tissue Dissociations Kits for specific tissues or optimization on the following pages.

Animal-free (AF) enzymes for research into biopharmaceutical and biomedical applications are crucial for mitigating the risks associated with animal-derived pathogens. A diverse range of enzymes, including key AF nucleases like DNases and RNase T1 & T2, Collagenases and other Proteases is part of our portfolio. These enzymes play pivotal roles in applications such as the isolation of primary and stem cells for autologous transplantation and artificial organ research. Ensuring the absence of animal-based components in these enzymes is essential to prevent potential contamination by mammalian pathogens.

AF enzymes are sourced from plant, fungal, bacterial, and other native or recombinant microbial origins. Their production involves the use of raw materials, media components, chemicals, buffers, and dedicated equipment that are entirely free of animal-derived materials.

The Bioprocess Grade Enzymes are specialized enzymes tailored to meet the stringent requirements of pharmaceutical and biomedical bioprocessing applications.

  • CLSS-2 - Collagenase, Type 2, filtered

    Cat.-Nr: LS004204

    Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain ≥ 50 milligrams per vial.

  • CLSS-2 - Collagenase, Type 2, filtered

    Cat.-Nr: LS004205

    Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and lyophilized in vials. =125 units per mg dry weight

  • CLSS-3 - Collagenase, Type 3, filtered

    Cat.-Nr: LS004206

    Collagenase, Type 3 (Code: CLS-3), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain ≥ 50 milligrams per vial.

  • CLSS-3 - Collagenase, Type 3, filtered

    Cat.-Nr: LS004208

    Collagenase, Type 3 (Code: CLS-3), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain ≥ 50 milligrams per vial.

  • CLSS-4 - Collagenase, Type 4, filtered

    Cat.-Nr: LS004209

    Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials. =160 units per mg dry weight

  • CLSS-4 - Collagenase, Type 4, filtered

    Cat.-Nr: LS004210

    Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain ≥ 50 milligrams per vial.

  • CLSS-4 - Collagenase, Type 4, filtered

    Cat.-Nr: LS004212

    Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain ≥ 50 milligrams per vial.

  • CLSS-5 - Collagenase, Type 5, filtered

    Cat.-Nr: LS005286

    Collagenase Type 5 (Code: CLSS-5), which is filtered through a 0.22 micron membrane and lyophilized in vials.

  • CLSS-5 - Collagenase, Type 5, filtered

    Cat.-Nr: LS005287

    Collagenase Type 5 (Code: CLSS-5), which is filtered through a 0.22 micron membrane and lyophilized in vials.

  • CLSS-5 - Collagenase, Type 5, filtered

    Cat.-Nr: LS005288

    Collagenase Type 5 (Code: CLS-5), which is filtered through a 0.22 micron membrane and lyophilized in vials.

  • Collagenase, Purified, Animal Free

    Cat.-Nr: LS005290

    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of... Read More

  • Collagenase, Purified, Animal Free

    Cat.-Nr: LS005292

    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of... Read More

  • Collagenase, Type D, Animal Free

    Cat.-Nr: LS004160

    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of... Read More

  • Collagenase, Type D, Animal Free

    Cat.-Nr: LS004162

    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of... Read More

  • Collagenase, Type D, Animal Free

    Cat.-Nr: LS004163

    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of... Read More

  • D2 - Dnase 2 - PDS Kit

    Cat.-Nr: LK003170

    A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial... Read More

  • D2 - DNase 2 - PDS-Kit

    Cat.-Nr: LK003172

    A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial... Read More

  • EBSS - Earle's Bal. Salt Sol. Vial PDS-Kit

    Cat.-Nr: LK003188

    Sterile Earle’s Balanced Salt Solution (EBSS) with calcium, magnesium, bicarbonate and phenol red, 1 x 100ml per package, as supplied in the Papain... Read More

  • ES - Elastase

    Cat.-Nr: LS002274

    2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22... Read More

  • ES - Elastase

    Cat.-Nr: LS002279

    2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron... Read More

  • ES - Elastase

    Cat.-Nr: LS002280

    2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron... Read More

  • ESFF - Elastase, purified

    Cat.-Nr: LS006363

    Chromatographically purified. A lyophilized powder.

  • ESFF - Elastase, purified

    Cat.-Nr: LS006365

    Chromatographically purified. A lyophilized powder.

  • ESL - Elastase, lyoph.

    Cat.-Nr: LS002290

    2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior... Read More

  • ESL - Elastase, lyoph.

    Cat.-Nr: LS002292

    2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to... Read More

  • ESL - Elastase, lyoph.

    Cat.-Nr: LS002294

    2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior... Read More

  • HBSS - Hank's Balanced Salt Solution

    Cat.-Nr: LK003210

    Sterile calcium and magnesium free Hank's balanced salt solution (CMFHBSS), pH 7.4, as supplied in the NCIS kit; 1 x 500 ml.

  • HBSS10 - Hank's Balanced Salt Solution 10x

    Cat.-Nr: LK002064

    Sterile calcium and magnesium free Hank's balanced salt solution, 10X CMF-HBSS concentrate, as supplied in the HIS kit; 1 x 500 ml

  • HIS - Hepatocyte Isolation System

    Cat.-Nr: LK002060

    Kit for performing five separate adult rat liver perfusions. Contains five single use CLSH enzyme vials, five single use DNase vials, 10X CMF-Hank's... Read More

  • HSE - Hyaluronidase

    Cat.-Nr: LS002592

    A partially purified, dialyzed, lyophilized powder.

  • HSE - Hyaluronidase

    Cat.-Nr: LS002594

    A partially purified, dialyzed, lyophilized powder.

  • HSEP - Hyaluronidase

    Cat.-Nr: LS005474

    Chromatographically purified. A dialyzed, lyophilized powder.

  • HSEP - Hyaluronidase

    Cat.-Nr: LS005475

    Chromatographically purified. A dialyzed, lyophilized powder.

  • HSEP - Hyaluronidase

    Cat.-Nr: LS005477

    Chromatographically purified. A dialyzed, lyophilized powder.

  • L15NK - Leibovitz L15 Media,NCIS

    Cat.-Nr: LK003250

    Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and... Read More

  • LBI - Trypsin Inhibitor, Lima Bean

    Cat.-Nr: LS002829

    Fraction III of the preparation described by Fraenkel-Conrat, et al., Arch. Biochem. Biophys., 37, 393 (1952). Supplied as a dialyzed, lyophilized... Read More

  • LBI - Trypsin Inhibitor, Lima Bean

    Cat.-Nr: LS002830

    Fraction III of the preparation described by Fraenkel-Conrat, et al., Arch. Biochem. Biophys., 37, 393 (1952). Supplied as a dialyzed, lyophilized... Read More

  • MOPS - 0.15m, MOPS Buffer, (MOPS)

    Cat.-Nr: LK002070

    0.15M MOPS, pH 7.5, 0.22u filtered. Buffer concentrate ( 75 ml) used to buffer the constituted Leibovitz L-15 media in Hepatocyte Isolation System.

  • NAH - Sodium bicarbonate, 7,5%

    Cat.-Nr: LK002069

    7.5% Sodium bicarbonate concentrate (NaHCO3), used to buffer the diluted CMF-HBSS; 1 x 100 ml

  • NCIS - Neonatal Cardiomyocyte Isolation Kit, 1 Kit

    Cat.-Nr: LK003300

    Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and... Read More

  • NCIS - Neonatal Cardiomyocyte Isolation Kit, 3 Kits

    Cat.-Nr: LK003303

    Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and... Read More

  • NPRO - Dispase (Neutr. Protease), purified

    Cat.-Nr: LS02100

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • NPRO - Dispase (Neutr. Protease), purified

    Cat.-Nr: LS02104

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • NPRO - Dispase (Neutr. Protease), purified

    Cat.-Nr: LS02106

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • NPRO2 - Neutral Protease, Partially purified

    Cat.-Nr: LS02109

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • NPRO2 - Neutral Protease, Partially purified

    Cat.-Nr: LS02111

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • NPRO2 - Neutral Protease, Partially purified

    Cat.-Nr: LS02110

    Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its... Read More

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003085

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003086

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003087

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.