Enzymes

Hydrolases represent a class central to hydrolytic cleavage reactions. Within this category, we cover a broad range, such as Glycosylases, Proteases, Phosphatases, Esterases, and Lipases. Within the group of Hydrolases, many enzymes are relevant for applications in the field of Molecular Biology, such as DNases, RNases, etc.

• Proteinases catalyse the cleavage (hydrolysis) of peptide bonds in proteins, leading to their breakdown into smaller peptides or amino acids. The enzyme class of Proteinases is often subdivided into Proteases and Peptidases. Proteases exhibit both endopeptidase (cutting within a peptide chain) and exopeptidase (cutting at the ends of a peptide chain) activity, while Peptidases primarily show exopeptidase activity. These enzymes play pivotal roles in cellular processes such as protein digestion, recycling, and cell cycle regulation. Their ability to break down proteins makes them indispensable tools for Proteomics studies, such as protein and peptide sequencing. Many proteases play pivotal roles in Tissue Dissociation for cell isolation, such as Collagenases, Trypsin and Papain.
• Esterases catalyse the hydrolysis of ester bonds in various organic compounds, including lipids, carbohydrates, and synthetic substrates. They play roles in lipid metabolism, signal transduction, and detoxification processes.
• Phosphatases catalyse the removal of phosphate groups from molecules, such as proteins, nucleotides, and sugars. They are involved in signal transduction, cell cycle regulation, and cellular metabolism.
• Glycosidases (also called glycoside hydrolases) catalyse the cleavage of glycosidic bonds, making them crucial for the repair of damaged DNA and the regulation of various cellular processes. Notably, we not only offer Lysozyme, a Glycosidase, but also the corresponding Lysozyme Substrate (Micrococcus lysodeikticus cells).
• Lipases are enzymes that catalyse the hydrolysis of lipids, breaking down triglycerides into fatty acids and glycerol. Their actions are essential for lipid digestion and metabolism in living organisms. Their ability to break down fats and oils makes them valuable tools for improving the efficiency of industrial processes and developing novel biotechnological applications.