DNase & Related Products

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DNase & Related Products

In molecular research, DNases play vital roles in DNA degradation and cleavage, while DNA and Histones are fundamental components in understanding genetic mechanisms and chromatin organization.

Deoxyribonuclease I (DNase I) is an enzyme that catalyzes the hydrolysis of phosphodiester bonds in DNA molecules. This results in the cleavage of DNA into smaller fragments. It is commonly used in molecular biology and biochemistry research for various applications, such as:

  • removal of contaminating genomic DNA from RNA preparations, e.g. prior to RT-PCR.
  • DNA fragmentation for DNA library construction.
  • preparation of single-cell suspensions by degradation of extracellular DNA released from lysed cells.

DNase I is mostly purified from bovine pancreas. These enzymes are available in different compositions, e.g. free of ribonuclease and protease (Code: DPRFS and DPRF), filtered (Code: DCLS and D2) and partially (Code: DSV, DP and DPB) or chromatographically (Code: DPFF and D) purified.

In addition, for sensitive applications and for industries where stringent quality and regulatory requirements must be met, purified recombinant DNase I products are available. These are produced in genetically engineered yeast (Pichia pastoris). Therefore, these DNase I  (Code: DR1, DR1S) are free of RNases, proteases and animal components (Animal Free, AF). The bioprocess grade DNase I (Code: DR2) is Animal Free.

In contrast, DNase II is purified from porcine spleen (Code: HDA, HDAC and HDACS). It has applications in studies focusing on apoptotic cell death and lysosomal DNA degradation research.

DNA-cellulose is a type of chromatography resin used in biochemistry and molecular biology for the purification and isolation of DNA-binding proteins. It consists of cellulose beads to which DNA molecules are covalently attached. DNA-cellulose chromatography takes advantage of the specific interactions between DNA and proteins, allowing researchers to separate proteins based on their affinity for DNA. This technique is commonly used to purify transcription factors, enzymes involved in DNA replication and repair, and other DNA-binding proteins for further study. We offer double-stranded (Code: DNACELDS) and sinlge-stranded DNA-cellulose (Code: DNACELSS) from calf thymus.

Furthermore, we offer DNA from

  • Calf Thymus: unaltered (Code: DNA),
  • Salmon Testes: unaltered (Code: SDNA) or fragmented (Code: SDNAD)
  • Phage Lambda: unaltered (Code: DNAL) and endonuclease digested with Hind III (Code: DNALHIND), EcoRI (Code: DNALECOR), and BstE II (Code: NDALBSTE)
  • E. coli (Code: DNAEC) and
  • Clostridium perfringens (Code: DNACP).

Histones, proteins located in eukaryotic cell nuclei, play a crucial role in packaging and organizing DNA into nucleosomes. Our product range includes Histones from calf thymus, available in dried (Code: H) and lyophilized (Code: HLY) forms. Additionally, we offer Nucleohistones from calf thymus (Code: NHL), where histone proteins are complexed with DNA.