Human Neonatal Dermal Fibroblasts Xeno-Free

Description

Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human pluripotent stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.

HDFn-XF are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HDFn-XF are quality tested in FibroLife® Xeno-Free Complete Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media containing non-human proteins or supplements.
Lifeline® HDFn-XF are not exposed to antimicrobials or phenol red when cultured in FibroLife® Xeno-Free Complete Medium, an advantage since these supplements can cause cell stress and ” masking effects” that may negatively impact experimental results.

500,000 cells per vial
Neonatal dermal fibroblasts are tested for:
Proliferation and morphology: Normal cell appearance for 15 population doublings
Cell viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma. Negative for bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Lifeline® documents that all materials used in the manufacture of products which are labeled “Xeno-Free” have never come into contact with material of animal (non-human) origin and are not of animal (non-human) origin.

For an overview of available human primary fibroblasts from different tissues and recommended culture media (as table or Fibroblast Product Family Flyer).

For an overview of available human primary cells from skin (neonatal & adult) and recommended culture media (click).

• SUPPLIER:

  Lifeline Cell Technology™

• STATUS:

  In Stock

• SIZE:

  500.000 cells/vial