Overview
- Features: Cell Imaging Reagents, solution
- Features:Dual-Color Fluorescence Staining Solution
Cytus™ Green Calcein AM, 100µl
Cat.-Nr.: 5468-100UL
Description
Cytus™ Green (calcein AM) is a cell-permeable, non-fluorescent compound that is enzymatically converted into intensely green fluorescent calcein by intracellular esterases in metabolically active cells (Ex/Em = 501/521 nm). Because this conversion requires intact cell membranes and active esterases, fluorescence is specific to live cells, with signal intensity correlating linearly to viable cell number. The resulting hydrophilic calcein is retained within the cytoplasm, providing strong, stable fluorescence suitable for quantitative
viability measurements.
Also availabel: The Cytus™ Green / Red Cell Viability Kit is a dual-color fluorescence assay designed for rapid and reliable assessment of live and dead cells. It combines Cytus™ Green Calcein AM for viable cells with Cytus™ Red Propidium Iodide for non-viable cells, enabling simultaneous discrimination within mixed populations.
| Parameter, Testing, and Method | Cytus™ Green #5468 |
| Live Cell Dye | Green (Cytus™ Green Calcein AM) |
| Stock Concentration | Green: 1 mM in DMSO |
| Stock Volume | Green: 100 µL |
| Appearance | Green: yellow hue; clear liquid |
| Sterility | Non-Sterile |
| Assay Type | Live dead cell viability |
| Cell Compatibility | Live and dead cells |
| Working Concentration | Green: 2 µM |
| Excitation/Emission (Green) | 501 / 521 nm |
| Storage Temperature | -20°C |
| Shelf Life | Minimum of 6 months from date of receipt |
SUPPLIER:
STATUS:
In Stock
SIZE:
100 µl, 500 tests
Cytus™ Green labels metabolically active cells.
Related Files
References
Cytus™ Red Propidium Iodide, 100µl