Overview
- Species: Other
- Additional Attributes: Media
- Specific Attributes: frozen, steril
- Features:kit -AlphaSTEM™ Basal Medium, AlphaSTEM™ Supplement 1, AlphaSTEM™ Supplement 2
AlphaSTEM® Naïve hPSC Medium
Cat.-Nr.: MN01500
Description
AlphaSTEM® Naïve hPSC Medium is a serum-free and feeder-free medium formulated for the generation and expansion of naïve pluripotent stem cells. Utilizing a naturally occurring growth factor, NME7AB , that is expressed in the earliest days of embryogenesis, AlphaSTEM® Naïve hPSC Medium fully supports the naïve state in human stem cells. The medium can also be used during reprogramming for high efficient generation of naïve induced pluripotent stem cells. These cells express pluripotency markers, as well as naïve markers, have two active X chromosomes (female lines) and differentiate down all three germlines.
SUPPLIER:
Minerva Biotechnologies
STATUS:
In Stock
SIZE:
500 ml
References
- A Primitive Growth Factor, NME7AB , Is Sufficient to Induce Stable Naïve State Human Pluripotency; Reprogramming in This Novel Growth Factor Confers Superior Differentiation. Carter MG, Smagghe BJ, Stewart AK, Rapley JA, Lynch E, Bernier KJ, Keating KW, Hatziioannou VM, Hartman EJ, Bamdad CC. Stem Cells. 2016 Apr;34(4):847-59. doi: 10.1002/stem.2261.
- MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. Smagghe BJ, Stewart AK, Carter MG, Shelton LM, Bernier KJ, Hartman EJ, Calhoun AK, Hatziioannou VM, Lillacci G, Kirk BA, DiNardo BA, Kosik KS, Bamdad C. PLoS One. 2013;8(3): e58601. doi: 10.1371/journal.pone.0058601.
- MUC1* is a determinant of trastuzumab (Herceptin) resistance in breast cancer cells. Fessler SP, Wotkowicz MT, Mahanta SK, Bamdad C. Breast Cancer Res Treat. 2009 Nov;118(1):113-24. doi: 10.1007/s10549-009-0412-3.
- A minimal fragment of MUC1 mediates growth of cancer cells. Mahanta S, Fessler SP, Park J, Bamdad C. PLoS One. 2008 Apr 30;3(4):e2054. doi: 10.1371/journal.pone.0002054.
- MUC1* mediates the growth of human pluripotent stem cells. Hikita ST, Kosik KS, Clegg DO, Bamdad C. PLoS One. 2008 Oct 3;3(10):e3312. doi: 10.1371/journal.pone.0003312.
TARGATT ™ Transgenic Kit (5 microinjections)