Overview
- Species: Synthetic
- Keywords: Reagents
- Specific Attributes: sterile
AlphaSTEM® Culture Substrate
Cat.-Nr.: MC01360
Description
AlphaSTEM® Culture Substrate is a mouse monoclonal antibody that provides a defined surface for feeder-free generation and expansion of naive pluripotent stem cells. AlphaSTEM® Culture Substrate binds to the MUC1* growth factor receptor that is expressed on all pluripotent human stem cells. When used with AlphaSTEM® Naive hPSC Medium, AlphaSTEM® Culture Substrate has been proven to supports the naïve state in human stem cells and can also be used during reprogramming for high efficient generation of naïve induced pluripotent stem cells.
SUPPLIER:
Minerva Biotechnologies
STATUS:
In Stock
SIZE:
360 µl
References
- A Primitive Growth Factor, NME7AB , Is Sufficient to Induce Stable Naïve State Human Pluripotency; Reprogramming in This Novel Growth Factor Confers Superior Differentiation. Carter MG, Smagghe BJ, Stewart AK, Rapley JA, Lynch E, Bernier KJ, Keating KW, Hatziioannou VM, Hartman EJ, Bamdad CC. Stem Cells. 2016 Apr;34(4):847-59. doi: 10.1002/stem.2261.
- MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. Smagghe BJ, Stewart AK, Carter MG, Shelton LM, Bernier KJ, Hartman EJ, Calhoun AK, Hatziioannou VM, Lillacci G, Kirk BA, DiNardo BA, Kosik KS, Bamdad C. PLoS One. 2013;8(3): e58601. doi: 10.1371/journal.pone.0058601.
- MUC1* is a determinant of trastuzumab (Herceptin) resistance in breast cancer cells. Fessler SP, Wotkowicz MT, Mahanta SK, Bamdad C. Breast Cancer Res Treat. 2009 Nov;118(1):113-24. doi: 10.1007/s10549-009-0412-3.
- A minimal fragment of MUC1 mediates growth of cancer cells. Mahanta S, Fessler SP, Park J, Bamdad C. PLoS One. 2008 Apr 30;3(4):e2054. doi: 10.1371/journal.pone.0002054.
- MUC1* mediates the growth of human pluripotent stem cells. Hikita ST, Kosik KS, Clegg DO, Bamdad C. PLoS One. 2008 Oct 3;3(10):e3312. doi: 10.1371/journal.pone.0003312.
AlphaSTEM® Differentiation Inducer