Overview
- Species: Human
- Keywords: Growth Factors
- Product Type: Leptin
- Donor / Source:Escherichia Coli.
- Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
- Purity:Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
- Amino acid sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
- Solubility:It is recommended to reconstitute the lyophilized Leptin Antagonist Quadruple Mutant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Stability:Lyophilized Leptin Antagonist Quadruple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
