Services & Products

From precise gene knockouts to seamless transgene insertions, from point mutations to conditional overexpression, and even complex gene fusions: your vision is limitless—and so are your options with the available Genome Editing Services.

Several genome-editing platforms offer cutting-edge solutions for precise and efficient genome modification, enabling the creation of customized induced pluripotent cell (iPSC) lines with a range of applications, including but not limited to:

• Gene Knockout: Frame shift, Gene disruption, site-specific large fragment deletion
• Gene Insertion : Large or small transgene insertion, Reporter / tag knock-in
• Single Nucleotide Polymorphisms (SNPs): Point mutations
• Overexpression: Conditional or inducible gene expression, Gene overexpression
• Gene Fusion: Translocation, Inversion

The turnaround time depends on the selected service and starting iPSCs, ranging from 6 weeks to 4 months.

TARGATT^TM Technology for stable Knock-in Modifications

TARGATT™ is a site-specific genome editing technology that enables highly efficient single-copy large DNA fragment insertion up to 20kb at a preselected safe harbor locus.

 CRISPR-Cas9: Revolutionizing Gene Editing in Stem Cells

CRISPR-Cas9 Technology enables or correct genetic mutations in iPSCs knock-out (KO/ conditional KO), knock-in (KI) or point mutation. As one of the earliest CRISPR/Cas9 genome editing services providers, Applied StemCell has the experience and optimized protocols for Rapid Automated Cell Line Editing (RACE™) in induced pluripotent stem cells.

SSelect™: Precise, One-Step Gene Insertion

SSelect™ enables highly efficient, one-step site-specific integration of large DNA sequences—up to 20 kb—into a designated safe harbor genomic locus using the proprietary SSelect™ integrase. Unlike many other gene-editing methods, SSelect™ is effective in both dividing and non-dividing cells, making it a versatile tool for autologous, allogeneic, and genomic therapeutic applications.

Accubase

AccuBase technology is developed to facilitate base editing. Accubase editing has very high safety profile and low off-target events compared to other CRISPR / base editing technologies.

MAD7 Gene Editing
MAD7 technology is a royalty-free alternative with gene-editing capabilities similar to CRISPR-Cas9. It is an attractive option for both commercial and research applications and is ideally suited for generating knock-out (KO), knock-in (KI), or point mutations.

Further along your drug discovery pipeline, cGMP compliant iPSC gene editing and associated services are also available. Contact us for more information.