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CRISPR-Cas9: Revolutionizing Gene Editing in Stem Cells
CRISPR-Cas9, discovered in 2012 by Jennifer Doudna and Emmanuelle Charpentier, is a revolutionary gene-editing tool that allows precise modification of DNA sequences in living organisms. This technology has enabled researchers to edit the genomes of iPSCs and other stem cells with unprecedented accuracy and efficiency.

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By harnessing CRISPR-Cas9, scientists can introduce or correct genetic mutations in iPSCs [knock-out (KO), knock-in (KI) or point mutation], making them valuable tools for modeling genetic diseases, studying developmental processes, and potentially developing personalized therapies. The combination of iPSCs and CRISPR technology holds great promise for advancing regenerative medicine and understanding the molecular mechanisms underlying various diseases.