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Home |Products|Matrix Proteins / Bioprinting|Adhesion Molecules & Peptides|Poly-L-Lysine Hydrobromide, Solution

Poly-L-Lysine Hydrobromide, Solution

Cat.-Nr.: 5048-50ML

Description

Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digest Poly-L-Lysine. For those cell types, Poly-D-Lysine, Catalog # 5049 should be used.

The molecular weight of Poly-L-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types.

Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Poly-L-Lysine is ideally suited for the coating of coverslips and other cell culture surfaces. Click here for a short protocol on how to coat your cell culture system.

 

  • SUPPLIER:

    Advanced BioMatrix

  • STATUS:

    In Stock

  • SIZE:

    50 ml

  • Overview
  • Related Files
  • References

Overview

  • Species: Synthetic
  • Keywords: Coating
  • Specific Attributes: Adhesion peptides
  • Features: animal-free, filtered (22 µm), solution, xeno-free
  • Features:0,1 mg/ml

Related Files

Datasheet
Certificate of Origin Coating Protocol Poly-L-Lysine

References

  • Påhlman, Lisa I., et al. "Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-β1." BMC pulmonary medicine 15.1 (2015): 19.
  • Li, Yun, et al. "Imaging pHluorin-tagged receptor insertion to the plasma membrane in primary cultured mouse neurons." JoVE (Journal of Visualized Experiments) 69 (2012): e4450.
  • Morand, David-Nicolas, et al. "Active nanofibrous membrane effects on gingival cell inflammatory response." Materials 8.10 (2015): 7217-7229.
  • Thuault, Sylvie, et al. "The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity." Molecular biology of the cell 27.17 (2016): 2653-2661.
  • Popielarski, Marcin, et al. "The role of Protein Disulfide Isomerase and thiol bonds modifications in activation of integrin subunit alpha11." Biochemical and biophysical research communications 495.2 (2018): 1635-1641.
  • Wu, Kun Lieh, et al. "Effects of culturing media on hepatocytes differentiation using Volvox sphere as co-culturing vehicle." Biochemical and biophysical research communications 458.3 (2015): 620-625.
  • Santio, Niina M., et al. "The PIM1 kinase promotes prostate cancer cell migration and adhesion via multiple signalling pathways." Experimental cell research 342.2 (2016): 113-124.

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