Targatt™ Large Knock-in Technology for iPSC Research
Large DNA knock-in made precise, scalable, and consistent
The proprietary TARGATT™ Large Knock-in Technology enables rapid, efficient, and site-specific integration of large DNA payloads (≥50 kb) into mammalian genomes. By targeting the well-characterized H11 safe harbor locus, TARGATT™ delivers single-copy, stable transgene insertion with highly reproducible expression – ideal for iPSC engineering, cell therapy development, and advanced research applications.
Key benefits:
- Single-copy integration at a predefined genomic site
- Large insert capacity (50 kb and beyond), including multi-gene constructs
- Site-specific recombination in the H11 safe harbor locus
- Consistent long-term expression
- Negligible off-target activity
This makes the TARGATT™ Knock-in Technology for iPSC research particularly attractive for cell and gene therapy developers and basic research on complex iPSC-based disease models.
How TARGATT™ technology works
TARGATT™ is based on an integrase–mediated recombination system using two short DNA recognition sequences: attB and attP.
Mechanism of action:
- The donor plasmid contains the DNA payload and one recombination site (attB or attP).
- The host genome harbors the complementary site, introduced as a TARGATT™ landing pad pre-engineered into the H11 safe harbor locus.
- The TARGATT™ integrase binds both sites and catalyzes a precise, site-specific recombination event.
- A single copy of the donor construct is stably integrated at the target locus.
- The attB and attP sites are destroyed during recombination, making the process unidirectional and irreversible, ensuring long-term genomic stability.
Available Products and Services:
AST-1602 TARGATT™ Female hiPSC Knock-in Kit
AST-9450 TARGATT™ hiPSC Knock-in Kit (GMP-Matching)
AST-9650 TARGATT™ Hypo hiPSC Knock-in Kit (Hypoimmunogenic)
AST-6012T TARGATT™ Site-specific Knock-in iPSC Service
AST-6001 TARGATT™ Master Cell Line Generation Service (Custom)
