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TARGATTTM Large Knock-in Technology for Mammalian Cell Line Development

Key Technological Advantages & Scientific Benefits

TARGATT™ Technology is a site-specific recombination system engineered for efficient, stable, and single-copy insertion of large gene fragments into defined mammalian safe harbor loci (such as the H11 locus). Unlike traditional viral or CRISPR-based knock-in methods, TARGATT™ utilizes a site-specific integrase and pre-engineered landing pad cell lines, overcoming common challenges associated with random integration and double-strand break-induced off-target effects.

  • Large Cargo Capacity (>50 kb): Accommodates large donor constructs – ranging from single genes to multi-gene circuits exceeding 50 kb –  making it ideal for complex protein co-expression, metabolic pathway engineering, and large antibody formats.
  • Defined Single-Copy Integration: Delivers precisely one gene copy per cell at a well-characterized genomic safe harbor locus (H11), ensuring predictable and long-term stable expression without copy-number variability.
  • High Knock-In Efficiency (Up to 100%): Reaches high knock-in efficiency post-selection, enabling rapid pool-based screening and high-throughput mammalian display libraries (up to 107 – 109 complexity) without extensive clonal isolation.
  • Negligible Off-Target Activity & Unidirectional Insertion: Eliminates double-strand break (DSB) risks and prevents cassette excision, providing a safer alternative to CRISPR and Cre/Flp-mediated recombination systems.
  • Accelerated Timelines (4 – 6 Weeks): Streamlines custom mammalian cell line engineering (including HEK293, CHO, and hiPSCs) for difficult-to-express proteins, membrane receptors, and inducible expression systems.
  • Clear IP for Commercialization: Fully defined intellectual property landscape suited for basic research, bioproduction, and therapeutic cell therapy applications.

Precise, scalable knock-ins for mammalian cells

Safe & site-specific: The integrase used is highly specific for the target site – the TARGATT™ landing pad, which doesn’t naturally occur in the human genome.

Large cargo: Integration is highly efficient even with inserts of 50 kb. Larger insertions can be accommodated with multiple, nested integrations.

Efficient: The engineered system is optimized for integration and, unlike CRISPR, doesn’t rely on host homologous recombination machinery.

Reliable expression: The H11 safe harbor locus delivers robust, reliable gene expression for rapid cell line engineering.

Automation-ready, pool-friendly & scalable: Highly efficient integration into the robust, reliably‑expressing H11 locus makes TARGATT™ compatible with pool‑based studies and automation.

Clear IP for commercialization:  Unlike the complexity of the CRISPR landscape, TARGATT™ licensing is straightforward.

How to use TARGATTTM Technology

Use TARGATT™ directly in your own lab with one of our kits, which include a cell line containing a TARGATT™ landing pad at the H11 safe harbor site, a donor plasmid for cloning your gene of interest, and the TARGATT™ integrase.

Or take advantage of full-service custom cell line engineering of mammalian cell lines – including hiPSCs – using TARGATT™ technology, CRISPR, and MAD7.

Contact us to discuss your project requirements and find the optimal solution.

Choose your product

TARGATT™ hiPSC Kits TARGATT™ HEK293 Kits TARGATT™ CHO Kits

Primary Applications of the TARGATTTM Technology

TARGATT™ technology addresses some of the most demanding challenges in cell line engineering – from expressing difficult proteins to building libraries in human cells and advancing gene therapy programs.

  • Protein & Antibody Engineering: Stable expression of challenging membrane proteins, bispecific antibodies, and multi-protein complexes at defined stoichiometries.
  • Mammalian Display & High-Throughput Screening: High-capacity library screening directly in human cells for biologically relevant hits.
  • Cell & Gene Therapy Development: High-efficiency safe-harbor gene editing in hiPSC lines and GMP-grade cell systems for scalable disease modeling and translational iPSC research.

For detailed information on TARGATT™ Technology and its applications,
– download the TARGATT™ Technology e-book or
– contact our experts to discuss your specific project needs.