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Home |Products|iPSC Research|MEFs|SNL Feeder Cells 76/7 Mouse Fibroblast STO Cell Line, P14, Mitomycin C Treated

SNL Feeder Cells 76/7 Mouse Fibroblast STO Cell Line, P14, Mitomycin C Treated

Cat.-Nr.: ASF-1327

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Description

Mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) generally require culture on feeder cells as well as medium containing Leukemia Inhibitory Factor (LIF) to maintain pluripotency and the capacity to self-renew.

The SNL 76/7 cell line, derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes, can be used as feeder cells for mouse and human ESC and iPSC culture. The SNL cells are sensitive to HAT selection (hypoxanthine, aminoprotein, and thymidine) and negative for HPRT (hypoxanthine guanine phosphoribosyl transferase).

Before use, SNL 76/7 cells must be mitotically inactivated by γ-irradiation or mitomycin C treatment.

  • SUPPLIER:

    Applied StemCell

  • STATUS:

    In Stock

  • SIZE:

    5.000.000 cells/vial

  • Overview
  • Related Files

Overview

  • Species: Mouse
  • Specific Attributes: frozen, SNL 76/7
  • Product Type: SNL 76/7
  • Features: cryopreserved, Mitomycin C-treated

Related Files

Datasheet

RELATED PRODUCTS


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  • Neo-resistant MEF, P2, untreated

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Link to: iPS Cells (iPSCs from PBMC, Caucasian, Female) Link to: iPS Cells (iPSCs from PBMC, Caucasian, Female) iPS Cells (iPSCs from PBMC, Caucasian, Female) Link to: PBMC – Complete Kit (25M) Link to: PBMC – Complete Kit (25M) PBMC – Complete Kit (25M)
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