Overview
- Species: Other
- Keywords: Bioinks for 3D Bioprinting
- Specific Attributes: Photoinitiator
- Features:Photoinitiator
Ruthenium Visible Light Photocrosslinking Kit
Cat.-Nr.: 5248-1KIT
Description
The Ruthenium visible light photocrosslinking kit is ideal for tissue engineering, cell culture, and bioprinting, where tuning the mechanical properties of the substrate is required. The kit provides enough photoinitiator for >200 mL of bioinks/hydrogels (following recommended concentrations).
Ruthenium is a photoinitiator that utilizes visible light photocrosslinking (400-450nm) to covalently crosslink free tyrosine and acryl groups.
Ruthenium photoinitiator has been tested on collagen type I, gelatin, silk fibroin, methacrylated hyaluronic acid, methacrylated gelatin, methacrylated collagen type I and PEGDA.
Ruthenium is water soluble and yields better cell cytocompatibility, and crosslinking efficiency.
Increasing Visible light intensity from 3-100 mW/cm2 using Ruthenium did not significantly decrease cell viability from 90%.
Increasing Ruthenium concentration by 10 times (10x) did not decrease cell viability from 90%.
Ruthenium is red/yellow/orange in color and will change the color of your solutions, hydrogels, or printed constructs.
This Ruthenium photocrosslinking kit is considered non-sterile. Adding antibiotics to your cell culture system, or sterile filtering is recommended. To sterile filter, resuspend the entire volume of Ruthenium and Sodium Persulfate (separately) and filter through small 0.2 micron button filters (separately). Use the sterile photoinitiator within 2 weeks.
SUPPLIER:
Advanced BioMatrix
STATUS:
In Stock
SIZE:
200 mg
References
- Parrish, J., Lim, K. S., Baer, K., Hooper, G. J., & Woodfield, T. B. F. (2018). A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models. Lab on a Chip. Advance online publication.
- Parker, J. D., Lim, K. S., Kieser, D. C., Woodfield, T. B. F., & Hooper, G. J. (2018). Is tranexamic acid toxic to articular cartilage when administered topically? What is the safe dose? Bone & Joint Journal, 100-B(3), 404-412.
- Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., Alcala-Orozco, C. R., van Dorenmalen, K. M. A., … Hooper, G. J., … Woodfield, T. B. F. (2018). Bio-resin for high resolution lithography-based biofabrication of complex cell laden constructs. Biofabrication, 10, 034101.
- Bertlein, S., Brown, G., Lim, K. S., Jungst, T., Boeck, T., Blunk, T., … Hooper, G. J., Woodfield, T. B. F., & Groll, J. (2017). Thiol-ene clickable gelatin: A platform bioink for multiple 3D biofabrication technologies. Advanced Materials, 29(44), 1703404.
- Mekhileri, N. V., Lim, K. S., Brown, G. C. J., Mutreja, I., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2017). Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs. Biofabrication. Advance online publication.
- Lim, Khoon S., et al. "New visible-light photoinitiating system for improved print fidelity in gelatin-based bioinks." ACS Biomaterials Science & Engineering 2.10 (2016): 1752-1762.
- Lim, K. S., Ramaswamy, Y., Roberts, J. J., Alves, M.-H., Poole-Warren, L. A., & Martens, P. J. (2015). Promoting cell survival and proliferation in degradable poly(vinyl alcohol)-tyramine hydrogels. Macromolecular Bioscience, 15(10), 1423-1432.
- Lim, K. S., Alves, M. H., Poole-Warren, L. A., & Martens, P. J. (2013). Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels. Biomaterials,34(29), 7097-7105.
- Green, R. A., Lim, K. S., Henderson, W. C., Hassarati, R. T., Martens, P. J., Lovell, N. H., & Poole-Warren, L. A. (2013). Living electrodes: Tissue engineering in the neural interface. Proceedings of the 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS). (pp. 6957-6960). IEEE.
Mebiol® Thermoreversible Hydrogel