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Home |Products|Matrix Proteins / Bioprinting|PhotoCol®-LAP, Methacrylated Collagen with LAP Kit

PhotoCol®-LAP, Methacrylated Collagen with LAP Kit

Cat.-Nr.: 5270-1KIT

Description

PhotoCol®-LAP is a methacrylated Type I collagen kit.These 3D collagen gels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated collagen, LAP photoinitiator, acetic acid and a neutralization solution.
Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.
The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.
The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.
The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS which allows blue light photocrosslinking of the collagen at 405 nm.
To sterile filter, resuspend and filter through a 0.2 micron button filter.

 

For an overview of our tunable 3D Hydrogels (photo-crosslinking) (click)

For an overview of our tunable 3D Hydrogels (chemical-crosslinking; click)

  • SUPPLIER:

    Advanced BioMatrix

  • STATUS:

    In Stock

  • SIZE:

    100 mg

  • Overview
  • Related Files
  • References

Overview

  • Species: Bovine
  • Additional Attributes: 3D Hydrogels, Bioinks for 3D Bioprinting, Collagen, Tunable Stiffness Hydrogels
  • Specific Attributes: methacrylated, steril, Telocollagen/acidic treatment, Type I
  • Packaging:Kit - PhotoCol®, Photoinitiator

Related Files

Datasheet
Certificate of Origin
Declaration of Material Source

References

  • Maloney, E. et al. Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. Micromachines 11, 208 (2020).
  • Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent. Experimental Eye Research 173, 188–193 (2018).
  • Kathryn E. Drzewiecki et al, A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine, TECHNOLOGY (2017).
  • Drzewiecki, K. E. et al. Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen. Langmuir 30, 11204–11211 (2014).
  • Gaudet, I. D. & Shreiber, D. I. Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel. Biointerphases 7, 25 (2012).
  • Izadifar, Mohammad, et al. "UV-assisted 3D bioprinting of nanoreinforced hybrid cardiac patch for myocardial tissue engineering." Tissue Engineering Part C: Methods 24.2 (2018): 74-88.
  • Stoecklin, Celine, et al. "A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues." Advanced Biosystems (2018): 1700237.
  • Mazzocchi, Andrea, et al. "Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments." Biofabrication (2018).
  • Nguyen, T. U., Watkins, K. E. & Kishore, V. Photochemically crosslinked cell‐laden methacrylated collagen hydrogels with high cell viability and functionality. Journal of Biomedical Materials Research Part A107,1541–1550 (2019).
  • Mazzocchi, A. et al.Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening. ACS Biomaterials Science & Engineering5,1937–1943 (2019).
  • Chhetri, A. et al. Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments. Current Protocols in Chemical Biology11,(2019).

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SNL 76/7 feeders, STO cell line, P12, untreatedPhotoCol®-RUT, Methacrylated Collagen with Ruthenium Kit
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