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Home |Products|Tissue Dissociation|Collagenase & Dispase|NPRO – Dispase (Neutr. Protease), purified

NPRO – Dispase (Neutr. Protease), purified

Cat.-Nr.: LS02106

Description

Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

 

Characteristics of Neutral Protease (Dispase®) from Bacillus polymyxa: 

Molecular Weight: 36 kda.

pH Optimum: Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0.

Specificity: Non-specific cleavage of peptide bonds containing leucine and phenylalanine.

Activators: Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+

Inhibitors: EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals

Application(s): Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.

Chromatographically purified

Other packing size available: 10 mg (#LS02100), 50 mg (#LS02104), and bulk (on request)

 

  • SUPPLIER:

    Worthington Biochemical

  • STATUS:

    In Stock

  • SIZE:

    250 mg

  • Overview

Overview

  • Species: B. polymyxa
  • Keywords: Proteolytic Enzymes
  • Specific Attributes: animal-free
  • Product Type: Dispase
  • Features: animal-free, lyophilized, purified
  • Donor / Source:Bacillus polymyxa
  • Activity:≥4 units per mg dry weight
  • Code:NPRO

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Link to: CD44 Anti-Human, FITC Link to: CD44 Anti-Human, FITC CD44 Anti-Human, FITC Link to: NPRO2 – Neutral Protease, Partially purified Link to: NPRO2 – Neutral Protease, Partially purified NPRO2 – Neutral Protease, Partially purified
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