Overview
- Species: Human
- Keywords: iPSC-derived cells
- iPSC: iPSC-Derived Motor Neurons
- Features: cryopreserved, male
- Cell Type: Neural Cells
- Features:contact us for more information
iPSC-derived Human Motor Neurons with Media
Cat.-Nr.: ASE-9701
Description
iPSC-derived Human Motor Neurons with Media (iPSCs from Fibroblasts, iPSC Line ASE-9211, African-American Male, Neonatal, Episomal)
Motor neurons (MN) are specialized neurons originating from the spinal cord, responsible for integrating signals from the brain and the muscles to control and coordinate voluntary and involuntary contraction of muscles and movement. Motor neuron degeneration or dysfunction has been implicated in severe and debilitating diseases such as spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), post-polio syndrome (PPS), and others. iPSC-derived motor neurons provide a convenient, consistent, reliable, and physiologically relevant source of cell lines to model diseases and to test novel therapies.
Applied StemCell has developed an efficient, integration-free, small-molecule-based method to differentiate high-quality motor neuron cells from human iPSCs. The proprietary differentiation protocol involves activating the Wnt pathway and SHH signaling, and inhibiting activin-nodal and BMP signaling. The differentiated motor neurons recapitulate the phenotype and functional parameters of primary and in vivo motor neurons.
We provide motor neurons differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from the fibroblasts of an African American male donor. These high-purity (>90%) cells show distinct neurite outgrowth in 2-7 days after thaw, express late-stage motor neuron precursor biomarkers HB9 and ChAT at day 2 after thaw, and express mature motor neuron biomarkers Tuj1 and MAP2 at day 5. The cells are provided as cryopreserved, late-stage precursors that mature in 5 days after recovery.
These iPSC-differentiated motor neurons can be used as control lines to compare phenotype and functionality of patient-derived and genome-edited iPSC-derived motor neurons, for co-culture models with other neurons, glial, and skeletal muscle cells, as well as for neurotoxicity and drug screening.
SUPPLIER:
Applied StemCell
STATUS:
In Stock
SIZE:
kit
iPSC-derived Human Motor Neurons with Media (iPSCs from Fibroblasts, iPSC Line ASE-9211, African-American Male, Neonatal, Episomal)
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