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Home |Products|Haemostasis & Thrombosis|Coagulation Proteins|Human Protein S

Human Protein S

Cat.-Nr.: HCPS-0090

Description

Protein S is a single chain vitamin K-dependent protein which is thought to function in both the coagulation and complement cascades (1,2). Approximately 60% of protein S circulating in plasma is complexed to C4b binding protein (C4BP). It has been suggested that -carboxyglutamic acid (gla) dependent binding of protein S to negatively charged phospholipids may function to concentrate C4BP at cell surfaces following injury. In the coagulation system, protein S functions as an anticoagulant cofactor protein. Activated protein C (APC) forms a 1:1 stoichiometric complex with protein S in the presence of Ca2+ and phospholipid vesicles (Kd = 6×10-9M) (3). In the presence of protein S, a moderate increase (3-10 fold) in the rate of factor Va and factor VIIIa inactivation by APC is observed in plasma and on the surface of unstimulated platelets. Protein S bound to C4BP does not possess APC cofactor activity. Recently, an additional binding protein which enhances the activity of protein S has been described (4). Proteolytic inactivation of protein S by thrombin has been proposed as a regulatory mechanism in this system. A single cleavage by thrombin abolishes protein S cofactor activity by removing an NH2-terminal peptide (Mr = 8000) which contains the gla domain. The domain structure of protein S is similar to that of the other vitamin K-dependant coagulation factors with the exception that protein S does not possess the catalytic triad. Protein S is a single chain protein containing 10 gla residues in the NH2-terminal domain and 4 epidermal growth factor (EGF) domains. Human protein S is isolated from fresh frozen plasma by a combination of conventional methods (9) and immunoaffinity chromatography as described by Jenny et al. (5). Purified protein S is supplied in 50% (vol/vol) glycerol/H2O and should be stored at -20°C. Purity is determined by SDS-PAGE analysis.

  • SUPPLIER:

    Haematologic TechnologiesProlytix

  • STATUS:

    In Stock

  • SIZE:

    100 µg

  • Overview

Overview

  • Species: Human
  • Keywords: Cofactors
  • Product Type: Cofactors
  • Formulation:50% glycerol/H2O/ 5 mM Calcium Chloride (vol/vol)
  • Purity:>95%
  • Localization:Plasma, free and complexed to C4BP
  • Concentration of plasma:10 µg/ml (free)
  • Mode of action:Cofactor for activated protein C
  • Molecular Weight:69000
  • Extinction Coeff:9,5
  • Isoelectric point:5.0-5.5
  • Structure:single chain, NH2-terminal gla domain, four EGF domains
  • Percent carbohydrate:0%
  • Post translationnal modifications:one β-hydroxyaspartate, ten gla residues, three β-hydroxyasparagine

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Link to: Human Protein Z Link to: Human Protein Z Human Protein Z Link to: Human Protein C Link to: Human Protein C Human Protein C
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